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Open AccessArticle

Biochemical Hydrogen Potential Tests Using Different Inocula

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DESTEC—Department of Energy, Systems, Territory and Construction engineering, University of Pisa, Largo Lucio Lazzarino, 56122 Pisa, Italy
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DIEF—Department of Industrial Engineering, University of Florence, via Santa Marta 3, 50139 Florence, Italy
*
Author to whom correspondence should be addressed.
Abbreviations: AD, anaerobic digestion; BHP, biochemical hydrogen potential; BMP, biochemical methane potential; DF, dark fermentation; FW, food waste; HCB, hydrogen consuming bacteria; HPB, hydrogen producing bacteria; LOD, limit of detection; OFMSW, organic fraction of municipal solid waste; SD, standard deviation; SHP, Specific hydrogen production; TKN, total kjeldahl nitrogen, TOC, Total organic Carbon; TS, total solids; TSC, tryptose sulphite cycloserine; TVS, total volatile solids; VFA, volatile fatty acid.
Sustainability 2019, 11(3), 622; https://doi.org/10.3390/su11030622
Received: 17 December 2018 / Revised: 21 January 2019 / Accepted: 21 January 2019 / Published: 24 January 2019
(This article belongs to the Special Issue Advances in Biorefining of Biowaste)
Four inocula collected from different operating facilities were tested in their hydrogenic performances by means of two biochemical hydrogen potential test set-ups using sucrose and food waste as substrates, with the aim of evaluating the influence of inoculum media in batch fermentative assays. The selected inocula were: activated sludge collected from the aerobic unit of a municipal wastewater treatment plant, digested sludge from an anaerobic reactor treating organic waste and cattle manure, digested sludge from an anaerobic reactor treating agroindustrial residues, and digested sludge from an anaerobic reactor of a municipal wastewater treatment plant. Test results, in terms of specific hydrogen production, hydrogen conversion efficiency, and volatile solids removal efficiency, were significantly dependent on the type of inoculum. Statistical analysis showed different results, indicating that findings were due to the different inocula used in the tests. In particular, assays performed with activated sludge showed the highest performances for both substrates and both experimental set-ups. View Full-Text
Keywords: biochemical hydrogen potential; inoculum; dark fermentation; anaerobic digestion; food waste; sucrose biochemical hydrogen potential; inoculum; dark fermentation; anaerobic digestion; food waste; sucrose
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Pecorini, I.; Baldi, F.; Iannelli, R. Biochemical Hydrogen Potential Tests Using Different Inocula. Sustainability 2019, 11, 622.

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