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Open AccessArticle

A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

1
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
2
Emanuel Institute of Biochemical Physics RAS, Moscow 119334, Russia
*
Author to whom correspondence should be addressed.
Sustainability 2019, 11(11), 3158; https://doi.org/10.3390/su11113158
Received: 22 April 2019 / Revised: 28 May 2019 / Accepted: 31 May 2019 / Published: 5 June 2019
(This article belongs to the Special Issue Advances in Biorefining of Biowaste)
The possible use of the concentration of intracellular adenosine triphosphate (ATP) as a parameter enabling quick and adequate evaluation of the metabolic activity of methanogenic cells was demonstrated in the work. This approach was used to analyze the effect of introducing potassium humate and fulvic acids (1–10 g/L) into media with four different methanogenic consortia producing biogas. The ATP concentration was analyzed by the bioluminescent luciferin–luciferase method at the beginning and end of the process. During the entire process, the biogas composition, biogas efficiency, and the kinetics of methanogenesis in the presence of humic compounds were determined. The increase in the concentration of potassium humate led to a decrease in the overall energy status of the cells and reduced methanogenesis efficiency. However, fulvic acids introduced into the media stimulated methanogenesis in half of the tested consortia, which was accompanied by an increase in ATP concentration in cell samples. So, a positive correlation between the metabolic activity of cells in biogas formation and the concentration of ATP was observed. ATP concentration control appears to be an attractive tool for finding compounds that suppress methanogenesis in landfills. View Full-Text
Keywords: ATP; biogas; methanogenesis; anaerobic consortia; potassium humate; fulvic acids ATP; biogas; methanogenesis; anaerobic consortia; potassium humate; fulvic acids
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Stepanov, N.; Senko, O.; Perminova, I.; Efremenko, E. A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis. Sustainability 2019, 11, 3158.

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