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Volume 11, December
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Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.

Microbiol. Res., Volume 11, Issue 1 (March 2020) – 5 articles

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Article
Influence of Pseudomonas spp. on Okra (Abelomuscus esculantaus L.) Growth Parameters and Antioxidant Activities under Soil Salinity
Microbiol. Res. 2020, 11(1), 8443; https://doi.org/10.4081/mr.2020.8443 - 05 Aug 2020
Cited by 1 | Viewed by 445
Abstract
In this study total twenty seven gram negative NaCl tolerant rhizobacterial strains were isolated from disturbed soils of Lucknow districts. The 3 selected sampling sites were Bijnor, Pasi Quila and Babasaheb Bhimrao Ambedkar University Campus, Lucknow, India. The isolates have been investigated for [...] Read more.
In this study total twenty seven gram negative NaCl tolerant rhizobacterial strains were isolated from disturbed soils of Lucknow districts. The 3 selected sampling sites were Bijnor, Pasi Quila and Babasaheb Bhimrao Ambedkar University Campus, Lucknow, India. The isolates have been investigated for different plant growth promoting and biochemical activities. Based on morphological and biochemical tests the total eight (8) rhizobacterial strains were designated as Pseudomonas spp. Among PGP traits, all 8 Pseudomonas strains were examined for Indole-3-Acetic Acid (IAA) production activity. The bacterialisolate IAA4 produces maximum 21.16 ± 3.24 μg mL–1 of IAA at 100 μg mL−1 tryptophan concentration among Pseudomonas strains. This strain show excellent P solubilization and N fixatation in qualitative assay. The higher amount of NaCl salt in soils positively reduces paddy plant physiology and growth parameters. The IAA4 inoculation in okra seeds significantly (p < 0.001) improved plant growth parameters, green pigments and modulate antioxidant activities under pot experimental study. Full article
Article
Effect of the Addition of Starter Cultures to Ground Meat for Hamburger Preparation
Microbiol. Res. 2020, 11(1), 8623; https://doi.org/10.4081/mr.2020.8623 - 04 Aug 2020
Cited by 2 | Viewed by 378
Abstract
The aim of this work was to study the effect of a selected lactic acid bacteria formulation on the microbiological characteristics and colour of beef hamburgers stored at different temperatures. All hamburgers were evaluated on day-0, day-1, day-3, and day-5 for the following [...] Read more.
The aim of this work was to study the effect of a selected lactic acid bacteria formulation on the microbiological characteristics and colour of beef hamburgers stored at different temperatures. All hamburgers were evaluated on day-0, day-1, day-3, and day-5 for the following microbiological parameters (Staphylococcus spp., enterococci, Lactococcus spp., Lactobacillus spp., total mesophilic aerobes, Pseudomonas spp., total coliforms) according to standard methods and for colorimetric measurements performed with Colorimeter - Digital Color Picker for iOS 10, under a 6500K light, with the CIELab system. All data (geometric mean for microbiological data) were elaborated with GraphPad InStat, 3.0b and GraphPad Prism 6.0d for Mac OS X. Two-way analysis of variance (ANOVA) followed by the Tukey's multiple comparisons test was performed. The analysis of the colour proved that the addition of LAB does not affect the natural colour of ground meat, avoiding the risk of hiding the spoilage or fastening it. The addition of the starter has preserved the colour stability throughout the preservation period, with the same behaviour both in the hamburgers stored at 4 °C and in those at 10 °C after thermal abuse or not. In conclusion, the application of the proposed LAB formulation maintains hamburgers quality standards and can be a potential tool to increase their shelf-life. Full article
Article
Assessment of Antimicrobial Activity of Cerumen (Earwax) and Antibiotics against Pathogenic Bacteria Isolated from Ear Pus Samples
Microbiol. Res. 2020, 11(1), 8565; https://doi.org/10.4081/mr.2020.8565 - 14 Jul 2020
Viewed by 508
Abstract
The present study is focused on the assessment of the antimicrobial activity of cerumen and antibiotics against bacteria isolated from ear pus samples. Thus, a total of 50 ear pus samples were collected from infected patients using sterile swabs and were screened using [...] Read more.
The present study is focused on the assessment of the antimicrobial activity of cerumen and antibiotics against bacteria isolated from ear pus samples. Thus, a total of 50 ear pus samples were collected from infected patients using sterile swabs and were screened using pure culture techniques. Total of 04 different bacterial isolates were identified while, the prevalence data revealed that Pseudomonas spp., were dominant (58%, n = 29) among isolated bacteria followed by Staphylococcus spp., (22%, n = 11), Escherichia coli (14%, n = 7) and Proteus spp., (6%, n = 3). Further, bioassay revealed that Pseudomonas spp., and Staphylococcus spp., were most sensitive to Clindamycin (94.73%) while displayed resistant to Ciprofloxacin and Ampicillin. Similarly, E. coli and Proteus spp., were most sensitive to Ciprofloxacin (92.8–95.21%) as compared to the other antibiotics. Moreover, antibacterial activity of cerumen was also assessed against test organisms and its maximum activity was observed against Pseudomonas spp., (90% equivalent to Clindamycin potency) and Staphylococcus spp., (60% equivalent to Amoxicillin potency) while least effective against E. coli (36%) and Proteus spp., (22%). Thus, it was concluded that the antibacterial activity of cerumen might be due to the presence of potential chemicals i.e., flavonoids and terpenoids.
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Article
Extremophilic Actinomycetes Isolated from Soil in Kazakhstan: Classification and Antimicrobial Activities
Microbiol. Res. 2020, 11(1), 8356; https://doi.org/10.4081/mr.2020.8356 - 14 Jul 2020
Viewed by 408
Abstract
Extremophilic actinomycetes species are capable of surviving in extreme environment and producing antibiotics. In this study, we hypothesize that extremophiles produce antimicrobial compounds that are potentially novel agent(s) effective against drug resistant pathogens. The goal of this study is to test inhibitory activity [...] Read more.
Extremophilic actinomycetes species are capable of surviving in extreme environment and producing antibiotics. In this study, we hypothesize that extremophiles produce antimicrobial compounds that are potentially novel agent(s) effective against drug resistant pathogens. The goal of this study is to test inhibitory activity of the extracts derived from extremophilic actinomycetes species against the most prevalent drug-resistant bacteria in Kazakhstani hospitals, and preliminarily analyze chemical composition of the active extracts. Actinomycetes species isolated from the soil of Kazakhstan were cultured in modified media mimicking extreme environment the species were isolated from. Antimicrobial compound(s) extracted with organic solvent were tested against conditionally pathogenic and multi-drug resistant pathogens Acinetobacter baumanni and Pseudomonas aeruginosa. Our study generated promising results regarding the potential discovery of novel components effective against drug resistant pathogens. Future studies will focus on further chemical analysis to identify the active component within these extremophilic extracts. Full article
Article
Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads
Microbiol. Res. 2020, 11(1), 8458; https://doi.org/10.4081/mr.2020.8458 - 20 Mar 2020
Cited by 1 | Viewed by 399
Abstract
In this study, Alpha amylase from Bacillus subtilis was immobilized by entrapment in Calcium Alginate beads (CA). To improve the properties of these beads, alginate was blended with Cellulosic Residue (CR) obtained from sorghumstarch extraction. The conditions of entrapment were optimized for a [...] Read more.
In this study, Alpha amylase from Bacillus subtilis was immobilized by entrapment in Calcium Alginate beads (CA). To improve the properties of these beads, alginate was blended with Cellulosic Residue (CR) obtained from sorghumstarch extraction. The conditions of entrapment were optimized for a maximum immobilization yield (Y%) by mathematical statistics, where the 23-full factorial design of experiments was used. The properties of calcium alginate beads were improved by comparing the activity of immobilized enzymes in the hydrolysis of starch. The activity of the immobilized enzyme by Calcium Alginate/Cellulosic Residue (CA/CR) was found to be higher than the Calcium Alginate method. Zn2+ and Cu2+ have inhibitory effects on both immobilized enzymes. The Bacillus subtilis immobilized in alginate can be reused for 7 cycles with 12.7 μmol of reduced sugars and 6 cycles for the entrapped enzyme in CA/CR with 30 μmol of reduced sugars. Full article
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