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Microbiology Research
Volume 11
Issue 1
10.4081/mr.2020.8458
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Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads

by
Abdallah Herizi
1,2,*,
Rachid Souilah
1,3,
Djaafar Djabali
1 and
Boubekeur Nadjemi
1
1
Department of Chemistry, Ecole Normale Supérieure de Kouba, Algeria
2
Faculty of Technology, Mohamed Boudiaf M’sila University, Algeria
3
Department of Physics, Ecole Normale Supérieure de Laghouat, Algeria
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2020, 11(1), 8458; https://doi.org/10.4081/mr.2020.8458
Submission received: 23 January 2020 / Revised: 23 January 2020 / Accepted: 8 February 2020 / Published: 20 March 2020

Abstract

In this study, Alpha amylase from Bacillus subtilis was immobilized by entrapment in Calcium Alginate beads (CA). To improve the properties of these beads, alginate was blended with Cellulosic Residue (CR) obtained from sorghumstarch extraction. The conditions of entrapment were optimized for a maximum immobilization yield (Y%) by mathematical statistics, where the 23-full factorial design of experiments was used. The properties of calcium alginate beads were improved by comparing the activity of immobilized enzymes in the hydrolysis of starch. The activity of the immobilized enzyme by Calcium Alginate/Cellulosic Residue (CA/CR) was found to be higher than the Calcium Alginate method. Zn2+ and Cu2+ have inhibitory effects on both immobilized enzymes. The Bacillus subtilis immobilized in alginate can be reused for 7 cycles with 12.7 μmol of reduced sugars and 6 cycles for the entrapped enzyme in CA/CR with 30 μmol of reduced sugars.
Keywords: alginate; cellulosic residue; α-amylase; enzyme immobilization; optimization alginate; cellulosic residue; α-amylase; enzyme immobilization; optimization

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MDPI and ACS Style

Herizi, A.; Souilah, R.; Djabali, D.; Nadjemi, B. Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads. Microbiol. Res. 2020, 11, 8458. https://doi.org/10.4081/mr.2020.8458

AMA Style

Herizi A, Souilah R, Djabali D, Nadjemi B. Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads. Microbiology Research. 2020; 11(1):8458. https://doi.org/10.4081/mr.2020.8458

Chicago/Turabian Style

Herizi, Abdallah, Rachid Souilah, Djaafar Djabali, and Boubekeur Nadjemi. 2020. "Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads" Microbiology Research 11, no. 1: 8458. https://doi.org/10.4081/mr.2020.8458

APA Style

Herizi, A., Souilah, R., Djabali, D., & Nadjemi, B. (2020). Optimization and Immobilization of Alpha-Amylase from Bacillus subtilis in Calcium Alginate and Calcium Alginate—Cellulosic Residue Beads. Microbiology Research, 11(1), 8458. https://doi.org/10.4081/mr.2020.8458

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