Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees
and Jianxiang Wu 1,2,*Round 1
Reviewer 1 Report
The article described two super-sensitive and specific anti- PPV monoclonal antibodies，MAb 13H4 and 4A11，which can be used for quickly and reliably detecting PPV in apricot. This present study is to broaden the knowledge about PPV detection. Especially, the dot-ELISA and CGICS assays could detect 22 PPV infection in apricot tree leaf crude extracts diluted up to 1:5120 and 1:6400 (w/v). The sensitivity of PPV detection by dot-ELISA and CGICS were increased a lot comparing with previously reported. Thus, this might be particularly useful for large scale PPV surveys in fields.
The manuscript is well written and meets the quality standard of journal.
Author Response
We would like to thank the respected reviewer 1 for your positive comments.
Reviewer 2 Report
In this paper, Guo et al reported two super-sensitive and specific anti-PPV monoclonal antibodies, which can be used in dot enzyme-linked immunosorbent assay (dot-ELISA) and colloidal gold immunochro
matographic strip (CGICS) assays. Interestingly, the authors found the sensitivity of the dot-ELISA using the PPV antibody is higher than that of RT-PCR. These antibodies and relative methods are useful for PPV monitoring. The paper is well writen and easily to follow.
Some minor points:
Line 53: " The 5′ end of PPV genome is covalently bind by a viral-linked protein (VPg)" has an grammar issue.
Line 208: delete "through differential centrifugation"
Line 210: 15 nm
Author Response
In this paper, Guo et al reported two super-sensitive and specific anti-PPV monoclonal antibodies, which can be used in dot enzyme-linked immunosorbent assay (dot-ELISA) and colloidal gold immunochromatographic strip (CGICS) assays. Interestingly, the authors found the sensitivity of the dot-ELISA using the PPV antibody is higher than that of RT-PCR. These antibodies and relative methods are useful for PPV monitoring. The paper is well written and easily to follow.
Our response: We would like to thank the respected reviewer 2 for your positive comments and useful suggestions.
Some minor points:
- Line 53: "The 5′ end of PPV genome is covalently bind by a viral-linked protein (VPg)" has a grammar issue.
Our response: Revised accordingly.
- Line 208: delete "through differential centrifugation"
Our response: Revised accordingly.
- Line 210: 15 nm
Our response: Revised accordingly.
