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Article

High Incidence of Strawberry Polerovirus 1 in the Czech Republic and Its Vectors, Genetic Variability and Recombination

1
Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre, Czech Academy of Sciences, 370 05 České Budějovice, Czech Republic
2
Laboratory for Molecular Biology, Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy 129, 508 01 Hořice, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editor: Sylvie German-Retana
Viruses 2021, 13(12), 2487; https://doi.org/10.3390/v13122487
Received: 20 November 2021 / Revised: 6 December 2021 / Accepted: 8 December 2021 / Published: 11 December 2021
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
In total, 332 strawberry plants from 33 different locations in the Czech Republic with or without disease symptoms were screened by RT-PCR for the presence of strawberry polerovirus 1 (SPV1) and five other viruses: strawberry mottle virus, strawberry crinkle virus, strawberry mild yellow edge virus, strawberry vein banding virus, and strawberry virus 1. SPV1 was detected in 115 tested strawberry plants (35%), including 89 mixed infections. No correlation between symptoms and the detected viruses was found. To identify potential invertebrate SPV1 vectors, strawberry-associated invertebrate species were screened by RT-PCR, and the virus was found in the aphids Aphis forbesi, A. gossypii, A. ruborum, A.sanquisorbae, Aulacorthum solani, Chaetosiphon fragaefolii, Myzus ascalonicus, and several other non-aphid invertebrate species. SPV1 was also detected in aphid honeydew. Subsequent tests of C. fragaefolii and A.gossypii virus transmission ability showed that at least 4 h of acquisition time were needed to acquire the virus. However, 1 day was sufficient for inoculation using C. fragaefolii. In conclusion, being aphid-transmitted like other tested viruses SPV1 was nevertheless the most frequently detected agent. Czech SPV1 isolates belonged to at least two phylogenetic clusters. The sequence analysis also indicated that recombination events influence evolution of SPV1 genomes. View Full-Text
Keywords: strawberry; mixed virus infection; RT-PCR; virus transmission strawberry; mixed virus infection; RT-PCR; virus transmission
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MDPI and ACS Style

Fránová, J.; Lenz, O.; Přibylová, J.; Čmejla, R.; Valentová, L.; Koloniuk, I. High Incidence of Strawberry Polerovirus 1 in the Czech Republic and Its Vectors, Genetic Variability and Recombination. Viruses 2021, 13, 2487. https://doi.org/10.3390/v13122487

AMA Style

Fránová J, Lenz O, Přibylová J, Čmejla R, Valentová L, Koloniuk I. High Incidence of Strawberry Polerovirus 1 in the Czech Republic and Its Vectors, Genetic Variability and Recombination. Viruses. 2021; 13(12):2487. https://doi.org/10.3390/v13122487

Chicago/Turabian Style

Fránová, Jana, Ondřej Lenz, Jaroslava Přibylová, Radek Čmejla, Lucie Valentová, and Igor Koloniuk. 2021. "High Incidence of Strawberry Polerovirus 1 in the Czech Republic and Its Vectors, Genetic Variability and Recombination" Viruses 13, no. 12: 2487. https://doi.org/10.3390/v13122487

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