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Next-Generation Sequencing Analysis of Cellular Response to Influenza B Virus Infection
Open AccessArticle

Experimental Infection Using Mouse-Adapted Influenza B Virus in a Mouse Model

1
Department of Development and Testing of Pharmacological Agents, Federal Research Center of Fundamental and Translational Medicine, 630117 Novosibirsk, Russia
2
Medical Department, Novosibirsk State University, 630090 Novosibirsk, Russia
3
Department of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint Petersburg, Russia
4
Goldberg Research Institute of Pharmacology and Regenerative Medicine Clinic, 634009 Tomsk, Russia
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(4), 470; https://doi.org/10.3390/v12040470
Received: 31 January 2020 / Revised: 3 April 2020 / Accepted: 16 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Non-A Influenza)
Every year, influenza B viruses (IBVs) contribute to annual illness, and infection can lead to serious respiratory disease among humans. More attention is needed in several areas, such as increasing virulence or pathogenicity of circulating B viruses and developing vaccines against current influenza. Since preclinical trials of anti-influenza drugs are mainly conducted in mice, we developed an appropriate infection model, using an antigenically-relevant IBV strain, for furtherance of anti-influenza drug testing and influenza vaccine protective efficacy analysis. A Victoria lineage (clade 1A) IBV was serially passaged 17 times in BALB/c mice, and adaptive amino acid substitutions were found in hemagglutinin (HA) (T214I) and neuraminidase (NA) (D432N). By electron microscopy, spherical and elliptical IBV forms were noted. Light microscopy showed that mouse-adapted IBVs caused influenza pneumonia on day 6 post inoculation. We evaluated the illness pathogenicity, viral load, and histopathological features of mouse-adapted IBVs and estimated anti-influenza drugs and vaccine efficiency in vitro and in vivo. Assessment of an investigational anti-influenza drug (oseltamivir ethoxysuccinate) and an influenza vaccine (Ultrix®, SPBNIIVS, Saint Petersburg, Russia) showed effectiveness against the mouse-adapted influenza B virus. View Full-Text
Keywords: influenza B virus; mouse-adapted; amino acid substitutions; pathogenicity; influenza model; virulence; antiviral drugs; influenza vaccine influenza B virus; mouse-adapted; amino acid substitutions; pathogenicity; influenza model; virulence; antiviral drugs; influenza vaccine
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MDPI and ACS Style

Prokopyeva, E.; Kurskaya, O.; Sobolev, I.; Solomatina, M.; Murashkina, T.; Suvorova, A.; Alekseev, A.; Danilenko, D.; Komissarov, A.; Fadeev, A.; Ramsay, E.; Shestopalov, A.; Dygai, A.; Sharshov, K. Experimental Infection Using Mouse-Adapted Influenza B Virus in a Mouse Model. Viruses 2020, 12, 470. https://doi.org/10.3390/v12040470

AMA Style

Prokopyeva E, Kurskaya O, Sobolev I, Solomatina M, Murashkina T, Suvorova A, Alekseev A, Danilenko D, Komissarov A, Fadeev A, Ramsay E, Shestopalov A, Dygai A, Sharshov K. Experimental Infection Using Mouse-Adapted Influenza B Virus in a Mouse Model. Viruses. 2020; 12(4):470. https://doi.org/10.3390/v12040470

Chicago/Turabian Style

Prokopyeva, Elena; Kurskaya, Olga; Sobolev, Ivan; Solomatina, Mariia; Murashkina, Tatyana; Suvorova, Anastasia; Alekseev, Alexander; Danilenko, Daria; Komissarov, Andrey; Fadeev, Artem; Ramsay, Edward; Shestopalov, Alexander; Dygai, Alexander; Sharshov, Kirill. 2020. "Experimental Infection Using Mouse-Adapted Influenza B Virus in a Mouse Model" Viruses 12, no. 4: 470. https://doi.org/10.3390/v12040470

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