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Differential Small RNA Responses against Co-Infecting Insect-Specific Viruses in Aedes albopictus Mosquitoes

Institut Pasteur, Viruses and RNA Interference Unit, CNRS Unité Mixte de Recherche 3569, 75724 Paris CEDEX 15, France
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Viruses 2020, 12(4), 468; https://doi.org/10.3390/v12040468
Received: 4 March 2020 / Revised: 15 April 2020 / Accepted: 17 April 2020 / Published: 21 April 2020
(This article belongs to the Section Insect Viruses)
The mosquito antiviral response has mainly been studied in the context of arthropod-borne virus (arbovirus) infection in female mosquitoes. However, in nature, both female and male mosquitoes are frequently infected with insect-specific viruses (ISVs). ISVs are capable of infecting the reproductive organs of both sexes and are primarily maintained by vertical transmission. Since the RNA interference (RNAi)-mediated antiviral response plays an important antiviral role in mosquitoes, ISVs constitute a relevant model to study sex-dependent antiviral responses. Using a naturally generated viral stock containing three distinct ISVs, Aedes flavivirus (AEFV), Menghai rhabdovirus (MERV), and Shinobi tetra virus (SHTV), we infected adult Aedes albopictus females and males and generated small RNA libraries from ovaries, testes, and the remainder of the body. Overall, both female and male mosquitoes showed unique small RNA profiles to each co-infecting ISV regardless of the sex or tissue tested. While all three ISVs generated virus-derived siRNAs, only MERV generated virus-derived piRNAs. We also studied the expression of PIWI genes in reproductive tissues and carcasses. In contrast to Piwi5-9, Piwi1-4 were abundantly expressed in ovaries and testes, suggesting that Piwi5-9 are involved in exogenous viral piRNA production. Together, our results show that ISV-infected Aedes albopictus produce viral small RNAs in a virus-specific manner and that male mosquitoes mount a similar small RNA-mediated antiviral response to that of females. View Full-Text
Keywords: Aedes albopictus; insect-specific viruses; co-infection; small interfering RNA; PIWI-interacting RNA; reproductive tissues; sex difference Aedes albopictus; insect-specific viruses; co-infection; small interfering RNA; PIWI-interacting RNA; reproductive tissues; sex difference
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Frangeul, L.; Blanc, H.; Saleh, M.-C.; Suzuki, Y. Differential Small RNA Responses against Co-Infecting Insect-Specific Viruses in Aedes albopictus Mosquitoes. Viruses 2020, 12, 468.

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