Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Cases and Sampling
2.2. Laboratory Investigation
2.3. Molecular Analyses
3. Results
3.1. Clinical Cases
3.2. Virus Isolation and Identification
3.3. Genome Characterization
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Id. | Lesions | Laboratory Outcome | ||
---|---|---|---|---|
Pestivirus | Chlamydophila abortus | |||
EO | CC | |||
1756/2017 (1+2) pool | (1) mummified (2) hairy fleece with abnormal yellow pigmentation | + | + | negative |
338710-1/2017 | hemorrhagic subcutaneous edema, serum hemorrhagic fluid in cavities | − | − | positive |
338710-2/2017 | + | + | positive | |
338710-3/2017 | + | + | positive | |
350462/2017 | no lesions | − | − | negative |
350530/2017 | no lesions | − | − | negative |
16235-1/2018 | hemorrhagic subcutaneous edema, serum hemorrhagic fluid in cavities | − | − | negative |
16235-2/2018 | hairy fleece with abnormal yellow pigmentation | + | − | negative |
30564-1/2018 | mummified | − | − | positive |
30564-2/2018 | mummified | − | − | positive |
37146/2018 | hemorrhagic subcutaneous edema, serum hemorrhagic fluid in cavities liver degeneration | − | − | positive |
PestivirusSpecies/Strain | 1- | 2- | 3- | 4- | 5- | 6- | 7- | 8- | 9- | 10- | 11- | 12- | 13- | 14- | 15- | 16- | 17- | 18- | 19- | 20- | 21- | 22- | 23- | 24- | 25- | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1. Ovine/Italy/1756/2017CC | XXX | |||||||||||||||||||||||||
2. Ovine/Italy/1756/2017EO | 100 | XXX | ||||||||||||||||||||||||
3. Ovine/Italy/16235-2/2018 | 99.5 | 99.5 | XXX | |||||||||||||||||||||||
4. Ovine/Italy/338710-2/2017 | 99.5 | 99.5 | 99.1 | XXX | ||||||||||||||||||||||
5. Ovine/Italy/338710-3/2017 | 94.8 | 94.8 | 95.3 | 94.3 | XXX | |||||||||||||||||||||
C | 6. AF091661_CSFV_strain_Brescia | 87.6 | 87.6 | 88.1 | 87.0 | 91.2 | XXX | |||||||||||||||||||
7. U90951_CSFV_strain_Alfort_A19 | 87.5 | 87.5 | 88.1 | 87.0 | 91.2 | 98.1 | XXX | |||||||||||||||||||
8. X87939_CSFV_strain_Alfort/187 | 87.5 | 87.5 | 88.1 | 87.0 | 91.2 | 98.1 | 100 | XXX | ||||||||||||||||||
9. FJ265020_CSFV_Sp01_Spain | 88.1 | 88.1 | 88.6 | 87.5 | 90.7 | 95.8 | 95.7 | 95.7 | XXX | |||||||||||||||||
nc | 10. AY159514.1_Pestivirus_strain_5440/99 | 95.3 | 95.3 | 95.8 | 94.8 | 98.6 | 90.7 | 90.7 | 90.7 | 90.2 | XXX | |||||||||||||||
nc | 11. AF462000-Tunisian_sheep_virus_35T | 86.4 | 86.4 | 87.0 | 85.8 | 85.9 | 82.0 | 82.5 | 82.5 | 81.5 | 86.4 | XXX | ||||||||||||||
G | 12. AB040131.1_Pestivirus_Giraffe_H138 | 72.0 | 72.0 | 72.6 | 71.2 | 71.4 | 68.5 | 71.2 | 71.2 | 69.1 | 72.7 | 79.4 | XXX | |||||||||||||
I | 13. KM408491_Pestivirus_Burdur/05_TR | 83.6 | 83.6 | 84.2 | 84.2 | 80.8 | 80.1 | 80.0 | 80.0 | 82.4 | 81.3 | 80.1 | 71.2 | XXX | ||||||||||||
14. NC_018713_Pestivirus_Aydin/04_TR | 81.9 | 81.9 | 82.5 | 82.5 | 80.2 | 81.8 | 80.6 | 80.6 | 82.9 | 82.0 | 81.9 | 76.4 | 92.9 | XXX | ||||||||||||
A | 15. CS114709_BVDV1_NADL | 71.8 | 71.8 | 71.1 | 71.0 | 71.1 | 71.9 | 71.7 | 71.7 | 69.6 | 70.4 | 69.0 | 63.9 | 74.1 | 73.5 | XXX | ||||||||||
16. AF091605_BVDV1_strain_Oregon_C24V | 70.5 | 70.5 | 71.2 | 69.7 | 72.5 | 71.9 | 71.8 | 71.8 | 70.3 | 73.1 | 69.0 | 63.8 | 72.9 | 71.0 | 93.3 | XXX | ||||||||||
B | 17. L32886_BVDV2_strain_890 | 70.3 | 70.3 | 70.9 | 69.5 | 66.1 | 64.5 | 65.7 | 65.7 | 65.6 | 66.1 | 68.5 | 68.0 | 65.9 | 62.5 | 72.4 | 69.8 | XXX | ||||||||
18. AY379547_BVDV2_strain_Giessen_6 | 71.6 | 71.6 | 72.3 | 70.8 | 68.9 | 66.6 | 67.8 | 67.8 | 67.6 | 68.9 | 68.5 | 70.0 | 71.3 | 69.3 | 74.9 | 72.4 | 90.9 | XXX | ||||||||
D | 19. AB122086-BDV_strain_Rudolph | 79.0 | 79.0 | 79.6 | 78.3 | 79.6 | 82.0 | 81.9 | 81.9 | 81.9 | 79.6 | 78.7 | 70.1 | 83.0 | 81.3 | 67.7 | 65.6 | 61.8 | 64.0 | XXX | ||||||
20. U65022_BDV_strain_Moredun_cp | 82.5 | 82.5 | 82.0 | 82.5 | 80.7 | 79.6 | 79.5 | 79.5 | 80.8 | 80.7 | 78.7 | 65.7 | 82.6 | 80.9 | 66.6 | 63.7 | 60.7 | 61.4 | 89.6 | XXX | ||||||
21. EF693989_BDV_isolate_90-F-6227 | 77.7 | 77.7 | 77.1 | 77.6 | 75.8 | 80.9 | 80.8 | 80.8 | 81.5 | 75.8 | 74.5 | 68.7 | 81.0 | 79.8 | 68.9 | 66.1 | 65.5 | 63.9 | 88.1 | 91.7 | XXX | |||||
22. AY738080_BDV_Chamois | 79.5 | 79.5 | 78.9 | 79.4 | 77.1 | 78.4 | 78.3 | 78.3 | 77.1 | 77.7 | 75.0 | 68.7 | 77.4 | 78.0 | 66.2 | 68.3 | 63.9 | 63.9 | 82.3 | 84.7 | 84.7 | XXX | ||||
23. EF693998_BDV_isolate_96-F-7624 | 79.5 | 79.5 | 78.9 | 79.4 | 78.3 | 76.5 | 76.4 | 76.4 | 78.3 | 77.7 | 74.3 | 65.6 | 77.0 | 73.9 | 68.3 | 67.0 | 63.3 | 61.7 | 86.8 | 87.9 | 87.4 | 88.5 | XXX | |||
24. EF693996_BDV_isolate_94-F-7446/1 | 80.9 | 80.9 | 80.3 | 80.8 | 81.5 | 80.9 | 80.8 | 80.8 | 80.9 | 80.3 | 80.1 | 70.0 | 78.0 | 78.6 | 70.3 | 68.9 | 65.3 | 63.9 | 89.2 | 91.8 | 90.3 | 89.6 | 90.1 | XXX | ||
25. KT072634_BDV_isolate_Italy-103761 | 78.2 | 78.2 | 78.8 | 77.4 | 77.5 | 80.0 | 79.9 | 79.9 | 81.8 | 77.5 | 74.1 | 66.3 | 78.9 | 76.5 | 68.9 | 68.9 | 64.4 | 63.6 | 89.6 | 88.0 | 86.4 | 84.0 | 88.5 | 88.1 | XXX |
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Sozzi, E.; Lavazza, A.; Gaffuri, A.; Bencetti, F.C.; Prosperi, A.; Lelli, D.; Chiapponi, C.; Moreno, A. Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy. Viruses 2019, 11, 744. https://doi.org/10.3390/v11080744
Sozzi E, Lavazza A, Gaffuri A, Bencetti FC, Prosperi A, Lelli D, Chiapponi C, Moreno A. Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy. Viruses. 2019; 11(8):744. https://doi.org/10.3390/v11080744
Chicago/Turabian StyleSozzi, Enrica, Antonio Lavazza, Alessandra Gaffuri, Fabio Carlo Bencetti, Alice Prosperi, Davide Lelli, Chiara Chiapponi, and Ana Moreno. 2019. "Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy" Viruses 11, no. 8: 744. https://doi.org/10.3390/v11080744