Plasmacytoid Dendritic Cells Contribute to the Production of IFN-β via TLR7-MyD88-Dependent Pathway and CTL Priming during Respiratory Syncytial Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. RSV Infection and pDC Depletion In Vivo
2.3. RSV Infection In Vitro and Cytokine Measurements
2.4. Preparation of Single Lung Cell Suspensions
2.5. Flow Cytometry
2.6. CD4+ and CD8+ T Cell Responses
2.7. RSV Titration
2.8. Statistics
3. Results
3.1. pDCs Produce Higher Levels of Interferon-β than DCs during RSV Infection In Vitro
3.2. pDCs and cDCs are the Interferon-β Producers during RSV Infection In Vivo
3.3. RSV Induces pDCs to Produce Interferon-β via the TLR7-MyD88- and IFNα1R-Dependent Pathway
3.4. pDCs Contribute to the Cytotoxic T Cell Response
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, T.H.; Oh, D.S.; Jung, H.E.; Chang, J.; Lee, H.K. Plasmacytoid Dendritic Cells Contribute to the Production of IFN-β via TLR7-MyD88-Dependent Pathway and CTL Priming during Respiratory Syncytial Virus Infection. Viruses 2019, 11, 730. https://doi.org/10.3390/v11080730
Kim TH, Oh DS, Jung HE, Chang J, Lee HK. Plasmacytoid Dendritic Cells Contribute to the Production of IFN-β via TLR7-MyD88-Dependent Pathway and CTL Priming during Respiratory Syncytial Virus Infection. Viruses. 2019; 11(8):730. https://doi.org/10.3390/v11080730
Chicago/Turabian StyleKim, Tae Hoon, Dong Sun Oh, Hi Eun Jung, Jun Chang, and Heung Kyu Lee. 2019. "Plasmacytoid Dendritic Cells Contribute to the Production of IFN-β via TLR7-MyD88-Dependent Pathway and CTL Priming during Respiratory Syncytial Virus Infection" Viruses 11, no. 8: 730. https://doi.org/10.3390/v11080730