Host Immune Response to ZIKV in an Immunocompetent Embryonic Mouse Model of Intravaginal Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Virus
2.3. Intravaginal Infection
2.4. Quantitative PCR (qPCR)
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primer Target | Forward | Reverse |
---|---|---|
IL-1β | AGCTTCAAATCTCGCAGCAG | TCTCCACAGCCACAATGAGT |
Il-6 | GACTGATGCTGGTGACAACC | AGACAGGTCTGTTGGGAGTG |
IL-18 | CTTCTGCAACCTCCAGCATC | GTGAAGTCGGCCAAAGTTGT |
Ccl2 | AACTGCATCTGCCCTAAGGT | CTGTCACACTGGTCACTCCT |
Cxcl1 | TGTGGGAGGCTGTGTTTGTA | ACGAGACCAGGAGAAACAGG |
Cxcl10 | AGCCATGGTCCTGAGACAAA | ACAGAGCTAGGACAGCCATC |
Mx1 | AGGCAGTGGTATTGTCACCA | AGACTTTGCCTCTCCACTCC |
TLR7 | ATGTCCTTGGCTCCCTTCTC | ACTGAGCCATGTCTCTTGCT |
TNFα | CTCATGCACCACCATCAAGG | ACCTGACCACTCTCCCTTTG |
zika virus 1087-1163 | CCGCTGCCCAACACAAG | CCACTAACGTTCTTTTGCAGACAT |
Dam | ZIKV | n Embryos | Malformed | Reabsorptions | Total Dead |
---|---|---|---|---|---|
Dam 1 | – | 7 | 0 | 0 | 0 |
Dam 2 | + | 10 | 0 | 1 | 1 |
Dam 3 | – | 10 | 0 | 0 | 0 |
Dam 4 | – | 7 | 1 | 0 | 1 |
Dam 5 | – | 4 | 0 | 0 | 0 |
Dam 6 | + | 10 | 0 | 0 | 0 |
Dam 7 | + | 6 | 0 | 1 | 1 |
ZIKV+ (n = 6) | ZIKV- (n = 8) | Total (n = 14) | |
---|---|---|---|
TLR7 | 6/6 | 4/8 | 10/14 |
Mx1 | 5/6 | 1/8 | 6/14 |
CCL2 | 6/6 | 2/8 | 8/14 |
CXCL1 | 6/6 | 0/8 | 6/14 |
CXCL10 | 4/6 | 0/8 | 4/14 |
IL-1β | 3/6 | 0/8 | 3/14 |
IL-18 | 5/6 | 3/8 | 8/14 |
TNF-α | 5/6 | 2/8 | 7/14 |
IL-6 | 5/6 | 1/8 | 6/14 |
Dam | ZIKV+ (n = 13) | ZIKV- (n = 1) | Total (n = 14) |
---|---|---|---|
TLR7 | 10/13 | 1/1 | 11/14 |
Mx1 | 5/13 | 0/1 | 5/14 |
CCL2 | 7/13 | 0/1 | 7/14 |
CXCL1 | 7/13 | 0/1 | 7/14 |
CXCL10 | 3/13 | 0/1 | 3/14 |
IL-1β | 2/13 | 0/1 | 2/14 |
IL-18 | 9/13 | 1/1 | 10/14 |
TNF-α | 6/13 | 0/1 | 6/14 |
IL-6 | 8/13 | 0/1 | 8/14 |
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Khaiboullina, S.F.; Lopes, P.; de Carvalho, T.G.; Real, A.L.C.V.; Souza, D.G.; Costa, V.V.; Teixeira, M.M.; Bloise, E.; Verma, S.C.; Ribeiro, F.M. Host Immune Response to ZIKV in an Immunocompetent Embryonic Mouse Model of Intravaginal Infection. Viruses 2019, 11, 558. https://doi.org/10.3390/v11060558
Khaiboullina SF, Lopes P, de Carvalho TG, Real ALCV, Souza DG, Costa VV, Teixeira MM, Bloise E, Verma SC, Ribeiro FM. Host Immune Response to ZIKV in an Immunocompetent Embryonic Mouse Model of Intravaginal Infection. Viruses. 2019; 11(6):558. https://doi.org/10.3390/v11060558
Chicago/Turabian StyleKhaiboullina, Svetlana F., Priscila Lopes, Toniana G. de Carvalho, Ana Luiza C. V. Real, Danielle G. Souza, Vivian V. Costa, Mauro M. Teixeira, Enrrico Bloise, Subhash C. Verma, and Fabiola M. Ribeiro. 2019. "Host Immune Response to ZIKV in an Immunocompetent Embryonic Mouse Model of Intravaginal Infection" Viruses 11, no. 6: 558. https://doi.org/10.3390/v11060558