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Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro

1,2, 1,2,3,4,*, 1,2, 1,2, 1,2, 1,2, 1,2,3,4 and 1,2,3,4,*
1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
3
Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China
4
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(6), 559; https://doi.org/10.3390/v11060559
Received: 3 April 2019 / Revised: 1 June 2019 / Accepted: 16 June 2019 / Published: 17 June 2019
(This article belongs to the Special Issue Flavivirus Replication and Pathogenesis)
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Abstract

Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus which is the leading causative agent of viral encephalitis in endemic regions. JEV NS3 is a component of the viral replicase complex and is a multifunctional protein. In this study, interleukin enhancer-binding factor 2 (ILF2) is identified as a novel cellular protein interacting with NS3 through co-immunoprecipitation assay and LC-MS/MS. The expression of ILF2 is decreased in JEV-infected human embryonic kidney (293T) cells. The knockdown of endogenous ILF2 by special short hairpin RNA (shRNA) positively regulates JEV propagation, whereas the overexpression of ILF2 results in a significantly reduced JEV genome synthesis. Further analysis revealed that the knockdown of ILF2 positively regulates viral replication by JEV replicon system studies. These results suggest that ILF2 may act as a potential antiviral agent against JEV infection. View Full-Text
Keywords: Japanese encephalitis virus; cellular interleukin enhancer-binding factor 2 (ILF2); antiviral activity; viral RNA translation Japanese encephalitis virus; cellular interleukin enhancer-binding factor 2 (ILF2); antiviral activity; viral RNA translation
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Cui, X.; Qian, P.; Rao, T.; Wei, Y.; Zhao, F.; Zhang, H.; Chen, H.; Li, X. Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro. Viruses 2019, 11, 559.

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