Mechanism of Action of Methotrexate Against Zika Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell and Virus Culture
2.2. Plaque Assay
2.3. Immunofluorescence Imaging
2.4. Cytotoxicity and Efficacy Study of MTX
2.5. Rescue of ZIKV Replication after MTX Treatment by Leucovorin
2.6. Rescue Effect of GAT Medium on ZIKV Replication and Cell Viability from Methotrexate
3. Results
3.1. Inhibition of ZIKV Replication with MTX
3.2. Cell Cytotoxicity and Antiviral Efficacy of Methotrexate
3.3. Relationship between MTX, Folate, and ZIKV Replication
3.4. Rescue Effect of GAT Medium on ZIKV Replication and Cell Viability during MTX Treatment
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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CC50 (µM) Values of MTX | ||
Assay | Vero cells | hNSCs |
CTG | 0.104 | 0.0163 |
CTF | >100 | >100 |
95% Cl (from CTG) | 0.0931–0.118 | 0.0148–0.0181 |
IC50 (µM) Values of MTX | ||
Assay | Vero cells | hNSCs |
Immunofluorescence | 0.245 | 0.334 |
95% Cl | 0.0908–0.463 | 0.1203–1.187 |
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Beck, S.; Zhu, Z.; Oliveira, M.F.; Smith, D.M.; Rich, J.N.; Bernatchez, J.A.; Siqueira-Neto, J.L. Mechanism of Action of Methotrexate Against Zika Virus. Viruses 2019, 11, 338. https://doi.org/10.3390/v11040338
Beck S, Zhu Z, Oliveira MF, Smith DM, Rich JN, Bernatchez JA, Siqueira-Neto JL. Mechanism of Action of Methotrexate Against Zika Virus. Viruses. 2019; 11(4):338. https://doi.org/10.3390/v11040338
Chicago/Turabian StyleBeck, Sungjun, Zhe Zhu, Michelli F. Oliveira, Davey M. Smith, Jeremy N. Rich, Jean A. Bernatchez, and Jair L. Siqueira-Neto. 2019. "Mechanism of Action of Methotrexate Against Zika Virus" Viruses 11, no. 4: 338. https://doi.org/10.3390/v11040338