Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

Fang, Qingli; Wang, Cui; Liu, Huan; Wu, Qingping; Liang, Siying; Cen, Minli; Dong, Qinting; Wei, Yingyi; Chen, Ying; Ouyang, Kang; Wei, Zuzhang; Huang, Weijian

doi:10.3390/v11121156

Open AccessArticle

Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

by

Qingli Fang

^†,

Cui Wang

^†,

Huan Liu

,

Qingping Wu

,

Siying Liang

,

Minli Cen

,

Qinting Dong

,

Yingyi Wei

,

Ying Chen

,

Kang Ouyang

,

Zuzhang Wei

^*

and

Weijian Huang

^*

College of Animal Science and Technology, Guangxi University, No.100 Daxue Road, Nanning 530004, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Viruses 2019, 11(12), 1156; https://doi.org/10.3390/v11121156

Submission received: 3 November 2019 / Revised: 6 December 2019 / Accepted: 11 December 2019 / Published: 13 December 2019

(This article belongs to the Special Issue Endemic and Emerging Swine Viruses)

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Abstract

Astroviral infection is considered to be one of the causes of mammalian diarrheal diseases. It has been shown that astrovirus infections cause varying degrees of diarrhea in turkeys and mice. However, the pathogenesis of porcine astrovirus is unknown. In this study, the virulence of a cytopathic porcine astrovirus (PAstV) strain (PAstV1-GX1) isolated from the PK-15 cell line was tested using seven-day-old nursing piglets. The results showed that PAstV1-GX1 infection could cause mild diarrhea, growth retardation, and damage of the villi of the small intestinal mucosa. However, all the above symptoms could be restored within 7 to 10days post inoculation (dpi). To evaluate the innate immunity response of PAstV in vivo, the alteration of inflammatory cytokine expression in piglets infected with PAstV1-GX1 was determined using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The mRNA expression levels of the IFNβ and ISG54 were found to be significantly elevated in virus-infected piglets. In contrast, expression of IFNλ was downregulated in piglets infected with PAstV1-GX1. In addition, the mRNA expression of the tight junction protein 1 and 2 and zonula occludin 1, which are associated with the intestinal barrier permeability, were affected after PAstV1 infection. The present study adds to our understanding of the pathogenic mechanism of PAstV and has established an animal model for further study of pig astrovirus infection.

Keywords: porcine astrovirus; pathogenesis; diarrhea; innate immunity

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MDPI and ACS Style

Fang, Q.; Wang, C.; Liu, H.; Wu, Q.; Liang, S.; Cen, M.; Dong, Q.; Wei, Y.; Chen, Y.; Ouyang, K.; et al. Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China. Viruses 2019, 11, 1156. https://doi.org/10.3390/v11121156

AMA Style

Fang Q, Wang C, Liu H, Wu Q, Liang S, Cen M, Dong Q, Wei Y, Chen Y, Ouyang K, et al. Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China. Viruses. 2019; 11(12):1156. https://doi.org/10.3390/v11121156

Chicago/Turabian Style

Fang, Qingli, Cui Wang, Huan Liu, Qingping Wu, Siying Liang, Minli Cen, Qinting Dong, Yingyi Wei, Ying Chen, Kang Ouyang, and et al. 2019. "Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China" Viruses 11, no. 12: 1156. https://doi.org/10.3390/v11121156

APA Style

Fang, Q., Wang, C., Liu, H., Wu, Q., Liang, S., Cen, M., Dong, Q., Wei, Y., Chen, Y., Ouyang, K., Wei, Z., & Huang, W. (2019). Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China. Viruses, 11(12), 1156. https://doi.org/10.3390/v11121156

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Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

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