Avian Flavivirus Enters BHK-21 Cells by a Low pH-Dependent Endosomal Pathway
Abstract
:1. Introduction
2. Material and Methods
2.1. Cells and Virus
2.2. Cell Infection and Drug Treatments
2.3. RNA Extraction and RT-qPCR
2.4. Cell Viability Assay
2.5. Confocal Microscopy
2.6. siRNA Transfections
2.7. Western Blotting
2.8. Statistical Analysis
3. Results
3.1. DTMUV Entry is pH Dependent
3.2. Lysosomotropic Agents Act at an Early Time Point in DTMUV Infection
3.3. Effect of Moderate Acidic pH Pretreatment on DTMUV
3.4. Effect of the Proteasome on DTMUV Entry
3.5. DTMUV Entry is Clathrin Dependent
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Baloch, A.S.; Liu, C.; Liang, X.; Liu, Y.; Chen, J.; Cao, R.; Zhou, B. Avian Flavivirus Enters BHK-21 Cells by a Low pH-Dependent Endosomal Pathway. Viruses 2019, 11, 1112. https://doi.org/10.3390/v11121112
Baloch AS, Liu C, Liang X, Liu Y, Chen J, Cao R, Zhou B. Avian Flavivirus Enters BHK-21 Cells by a Low pH-Dependent Endosomal Pathway. Viruses. 2019; 11(12):1112. https://doi.org/10.3390/v11121112
Chicago/Turabian StyleBaloch, Abdul Sattar, Chunchun Liu, Xiaodong Liang, Yayun Liu, Jing Chen, Ruibing Cao, and Bin Zhou. 2019. "Avian Flavivirus Enters BHK-21 Cells by a Low pH-Dependent Endosomal Pathway" Viruses 11, no. 12: 1112. https://doi.org/10.3390/v11121112