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Open AccessArticle

Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells

1
Host-Pathogen Interactions, Paul-Ehrlich-Institut, 63225 Langen, Germany
2
Institute of Virology, Medical Faculty “Carl Gustav Carus”, Technische Universität Dresden, 01307 Dresden, Germany
3
CRTD/DFG-Center for Regenerative Therapies, Technische Universität Dresden, 01307 Dresden, Germany
4
Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 München, Germany
5
German Center for Infection Research (DZIF), 63225 Langen, Germany
6
Immunity and Pathogenesis Program, SBP Medical Discovery Institute, La Jolla, CA 92037, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this study.
Viruses 2019, 11(12), 1095; https://doi.org/10.3390/v11121095
Received: 30 September 2019 / Revised: 21 November 2019 / Accepted: 22 November 2019 / Published: 26 November 2019
(This article belongs to the Special Issue Spumaretroviruses)
Foamy viruses (FVs) belong to the Spumaretrovirinae subfamily of retroviruses and are characterized by unique features in their replication strategy. This includes a reverse transcription (RTr) step of the packaged RNA genome late in replication, resulting in the release of particles with a fraction of them already containing an infectious viral DNA (vDNA) genome. Little is known about the immune responses against FVs in their hosts, which control infection and may be responsible for their apparent apathogenic nature. We studied the interaction of FVs with the innate immune system in myeloid cells, and characterized the viral pathogen-associated molecular patterns (PAMPs) and the cellular pattern recognition receptors and sensing pathways involved. Upon cytoplasmic access, full-length but not minimal vector genome containing FVs with active reverse transcriptase, induced an efficient innate immune response in various myeloid cells. It was dependent on cellular cGAS and STING and largely unaffected by RTr inhibition during viral entry. This suggests that RTr products, which are generated during FV morphogenesis in infected cells, and are therefore already present in FV particles taken up by immune cells, are the main PAMPs of FVs with full-length genomes sensed in a cGAS and STING-dependent manner by the innate immune system in host cells of the myeloid lineage. View Full-Text
Keywords: retrovirus; foamy virus; spumavirus; innate sensing; cGAS; STING retrovirus; foamy virus; spumavirus; innate sensing; cGAS; STING
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Bergez, M.; Weber, J.; Riess, M.; Erdbeer, A.; Seifried, J.; Stanke, N.; Munz, C.; Hornung, V.; König, R.; Lindemann, D. Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells. Viruses 2019, 11, 1095.

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