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Viruses 2018, 10(11), 579; https://doi.org/10.3390/v10110579

Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)

Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, Viikinkaari 9, University of Helsinki, FI-00014 Helsinki, Finland
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Received: 21 September 2018 / Revised: 17 October 2018 / Accepted: 19 October 2018 / Published: 23 October 2018
(This article belongs to the Special Issue Viruses of Microbes V: Biodiversity and Future Applications)
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Abstract

Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer. Controlled dissociation of virions provides important information on virion composition, interactions, and stoichiometry of virion components, as well as their possible role in virus life cycles. Dissociation of viruses can be achieved by using various chemicals, enzymatic treatments, and incubation conditions. Asymmetrical flow field-flow fractionation (AF4) is a gentle method where the separation is based on size. Here, we applied AF4 for controlled dissociation of enveloped bacteriophage φ6. Our results indicate that AF4 can be used to assay the efficiency of the dissociation process and to purify functional subviral particles. View Full-Text
Keywords: enveloped virus; bacteriophage; virus disassembly; subviral particles; field-flow fractionation; size-based separation; dsRNA virus enveloped virus; bacteriophage; virus disassembly; subviral particles; field-flow fractionation; size-based separation; dsRNA virus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Eskelin, K.; Poranen, M.M. Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4). Viruses 2018, 10, 579.

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