Next Article in Journal
Comparison of the Properties of Alkali-Phenolic Binder in Terms of Selection of Molding Sand for Steel Castings
Previous Article in Journal
Correlation of Morphology and In-Vitro Degradation Behavior of Spray Pyrolyzed Bioactive Glasses
Previous Article in Special Issue
Human Mesenchymal Stem Cell Response to Lactoferrin-based Composite Coatings
Open AccessFeature PaperReview

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

1
Victor Babes National Institute of Pathology, Biochemistry-Proteomics Department, 050096 Bucharest, Romania
2
Department Pharmaceutical Biotechnology, National Institute for Chemical-Pharmaceutical R&D, 031299, Bucharest, Romania
3
National Institute of Materials Physics, 077125 Magurele, Romania
4
Army Centre for Medical Research, 010195 Bucharest, Romania
5
Department of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050047 Bucharest, Romania
6
Cantacuzino National Medico-Military Institute for Research and Development, 050096 Bucharest, Romania
7
Cajal Institute, Titu Maiorescu University, 004051 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Materials 2019, 12(22), 3704; https://doi.org/10.3390/ma12223704 (registering DOI)
Received: 30 September 2019 / Revised: 29 October 2019 / Accepted: 5 November 2019 / Published: 10 November 2019
(This article belongs to the Special Issue Biomaterials and Implant Biocompatibility)
Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions. View Full-Text
Keywords: bioceramics; in vitro testing; hydroxyapatite; angiogenesis; osteogenesis; signaling pathways; microRNA bioceramics; in vitro testing; hydroxyapatite; angiogenesis; osteogenesis; signaling pathways; microRNA
Show Figures

Figure 1

MDPI and ACS Style

Albulescu, R.; Popa, A.-C.; Enciu, A.-M.; Albulescu, L.; Dudau, M.; Popescu, I.D.; Mihai, S.; Codrici, E.; Pop, S.; Lupu, A.-R.; Stan, G.E.; Manda, G.; Tanase, C. Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications. Materials 2019, 12, 3704.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop