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Open AccessArticle

Human Mesenchymal Stem Cell Response to Lactoferrin-based Composite Coatings

1
Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania
2
Department of Biochemistry and Molecular Biology, University of Bucharest, Faculty of Biology, 91–95 Splaiul Independentei, 050095 Bucharest, Romania
3
National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor, 077125 Magurele, Romania
4
Faculty of Physics, University of Bucharest, RO-077125 Magurele, Romania
5
School of Engineering and Design, Brunel University, London UB8 3PH, UK
*
Authors to whom correspondence should be addressed.
Materials 2019, 12(20), 3414; https://doi.org/10.3390/ma12203414
Received: 27 August 2019 / Revised: 30 September 2019 / Accepted: 16 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Biomaterials and Implant Biocompatibility)
The potential of mesenchymal stem cells (MSCs) for implantology and cell-based therapy represents one of the major ongoing research subjects within the last decades. In bone regeneration applications, the various environmental factors including bioactive compounds such as growth factors, chemicals and physical characteristics of biointerfaces are the key factors in controlling and regulating osteogenic differentiation from MSCs. In our study, we have investigated the influence of Lactoferrin (Lf) and Hydroxyapatite (HA) embedded within a biodegradable PEG-PCL copolymer on the osteogenic fate of MSCs, previous studies revealing an anti-inflammatory potential of the coating and osteogenic differentiation of murine pre-osteoblast cells. The copolymer matrix was obtained by the Matrix Assisted Pulsed Laser Evaporation technique (MAPLE) and the composite layers containing the bioactive compounds (Lf, HA, and Lf-HA) were characterised by Scanning Electron Microscopy and Atomic Force Microscopy. Energy-dispersive X-ray spectroscopy contact angle and surface energy of the analysed coatings were also measured. The characteristics of the composite surfaces were correlated with the viability, proliferation, and morphology of human MSCs (hMSCs) cultured on the developed coatings. All surfaces were found not to exhibit toxicity, as confirmed by the LIVE/DEAD assay. The Lf-HA composite exhibited an increase in osteogenic differentiation of hMSCs, results supported by alkaline phosphatase and mineralisation assays. This is the first report of the capacity of biodegradable composite layers containing Lf to induce osteogenic differentiation from hMSCs, a property revealing its potential for application in bone regeneration. View Full-Text
Keywords: mesenchymal stem cells; osteogenic differentiation; lactoferrin; polymer composite mesenchymal stem cells; osteogenic differentiation; lactoferrin; polymer composite
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Icriverzi, M.; Bonciu, A.; Rusen, L.; Sima, L.E.; Brajnicov, S.; Cimpean, A.; Evans, R.W.; Dinca, V.; Roseanu, A. Human Mesenchymal Stem Cell Response to Lactoferrin-based Composite Coatings. Materials 2019, 12, 3414.

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