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Open AccessArticle

The Effect of Plasma Pretreatment and Cross-Linking Degree on the Physical and Antimicrobial Properties of Nisin-Coated PVA Films

1
Centre of polymer systems, Tomas Bata University, Trida Tomase Bati 5678, 76001 Zlin, Czech Republic
2
Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 267/2, 63711 Brno, Czech Republic
3
Department of Fat, Surfactants and Cosmetics Technology, Faculty of Technology, Tomas Bata University, Vavrečkova 275, 76001 Zlin, Czech Republic
*
Author to whom correspondence should be addressed.
Materials 2018, 11(8), 1451; https://doi.org/10.3390/ma11081451
Received: 13 July 2018 / Revised: 12 August 2018 / Accepted: 13 August 2018 / Published: 16 August 2018
(This article belongs to the Special Issue Surface Modification to Improve Properties of Materials)
Stable antimicrobial nisin layers were prepared on the carrying medium-polyvinyl alcohol (PVA) films, crosslinked by glutaric acid. Surface plasma dielectric coplanar surface barrier discharge (DCSBD) modification of polyvinyl alcohol was used to improve the hydrophilic properties and to provide better adhesion of biologically active peptide-nisin to the polymer. The surface modification of films was studied in correlation to their cross-linking degree. Nisin was attached directly from the salt solution of the commercial product. In order to achieve a stable layer, the initial nisin concentration and the following release were investigated using chromatographic methods. The uniformity and stability of the layers was evaluated by means of zeta potential measurements, and for the surface changes of hydrophilic character, the water contact angle measurements were provided. The nisin long-term stability on the PVA films was confirmed by tricine polyacrylamide gel electrophoresis (SDS-PAGE) and by antimicrobial assay. It was found that PVA can serve as a suitable carrying medium for nisin with tunable properties by plasma treatment and crosslinking degree. View Full-Text
Keywords: antimicrobial film; nisin; physical properties; plasma treatment polyvinyl alcohol; surface characterization antimicrobial film; nisin; physical properties; plasma treatment polyvinyl alcohol; surface characterization
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MDPI and ACS Style

Kolarova Raskova, Z.; Stahel, P.; Sedlarikova, J.; Musilova, L.; Stupavska, M.; Lehocky, M. The Effect of Plasma Pretreatment and Cross-Linking Degree on the Physical and Antimicrobial Properties of Nisin-Coated PVA Films. Materials 2018, 11, 1451.

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