The Promotive Effect of Cyanobacteria and Chlorella sp. Foliar Biofertilization on Growth and Metabolic Activities of Willow (Salix viminalis L.) Plants as Feedstock Production, Solid Biofuel and Biochar as C Carrier for Fertilizers via Torrefaction Process
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Treatments
2.3. Assessments of Physiological Parameters of Biofertilized Plants
2.4. Proximate Analysis
2.5. Fuel Characteristics
2.5.1. Caloric Value
- Thermogravimetric analysis TG, DTA, TGA TG-FTiR, and TG-MS carbonization process for untreated and carbonized willow combustion;
- Proximate and technical analysis of the willow torrefaction process and final products;
- Gas analysis of torgas formed as a result of carbonization: FTiR TG-MS analysis;
- Fuel analysis (caloric value, ash content, moisture content) of willow carbonization process products;
- SEM-EDS ash analysis of carbonized Salix viminalis L. after combustion.
2.5.2. Ash Characteristics of the Torrefied Salix viminalis L. Biomass
2.6. Analyses of Willow Energy Properties and Torrefied Willow Charactersitics
3. Statistical Analysis
4. Results and Discussion
4.1. Impact of Cyanobacteria and Chlorella sp. on Growth of Willow
4.2. Effect of Cyanobacteria and Chlorella sp. on Permeability of Cytomembranes and Physiological Activity in Willow Plants
4.3. Effect of Cyanobacteria and Algae on Composition and Energy Value of Willow Biomass
4.4. Circulation of By-Products (Nutrients) in the Closed Circular Production of Salix viminalis L. as a Source of Energy Fuel in Four Closed Cycles
5. Conclusions
- The triple foliar biofertilization with Cyanobacteria (Microcystis aeruginosa MKR 0105, Anabaena sp. PCC 7120) and green algae (Chlorella sp.) significantly increase the willow plant development, and several metabolic events have a key influence on plant growth and biomass yielding, of which energy value can be improved in the torrefaction process.
- The use of non-toxic Cyanobacteria and Chlorella sp. is cost-efficient and environmentally friendly and increases productivity of willow crops when used as biofuel for energy production.
- New physiological possibilities concerning enhancement of natural plant defense is considered one of the most promising strategies for ecological and integrated protection of energy crops cultivated on large areas.
- The developed technology for the production of willow biomass by means of fertilization with algae and increasing its energy properties in the thermo-chemical process enables the production of environmentally friendly energy as an alternative to that obtained from fossil fuels.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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N | P | K | Ca | Mg | Fe | Mn | Cu | Zn | B | Dry Mass |
---|---|---|---|---|---|---|---|---|---|---|
mg kg−1 Dry Weight | % | |||||||||
1.01 | 930 | 3829 | 25,734 | 1553 | 656 | 45.1 | 23.5 | 29.0 | 60.1 | 25.3 |
Applied Preparate, Cyanobacteria and Algae | Day and Month of Measurements | ||||
---|---|---|---|---|---|
1 June | 11 June | 22 June | 12 July | 3 August | |
Control | 1.2 a * | 2.2 a | 2.3 a | 2.5 a | 2.6 a |
Bio-Algeen S90 | 1.4 b | 2.3 b | 2.7 b | 2.9 b | 2.9 b |
Microcistis aeruginosa MKR 0105 | 1.5 b | 2.5 d | 3.1 cd | 3.7 d | 3.7 c |
Chlorella sp. | 1.5 b | 2.4 c | 3.0 c | 3.4 c | 3.6 c |
Anabaena sp. PCC 7120 | 1.4 b | 2.4 c | 2.9 c | 3.4 c | 3.5 c |
Environmental sample | 1.3 a | 2.3 b | 2.7 b | 2.9 b | 2.9 b |
LSD 0.05 ** | 0.1 | 0.08 | 0.1 | 0.2 | 0.2 |
Applied Preparate, Cyanobacteria and Algae | Activity of Selected Enzymes | ||||
---|---|---|---|---|---|
Phosphorylase Acid (mU g−1 f.w.) | Phosphorylase Alkaline (mU g−1 f.w.) | RNase (mU g−1 f.w.) | Dehydrogenases (mg Formazan · g leaf −1) | Nitrate Reductase (μmol NO2 g−1 f.w. h−1) | |
Control | 0.54 a * | 0.14 a | 2.6 a | 0.77 a | 0.90 a |
Bio-Algeen S90 | 0.76 b | 0.25 b | 3.75 b | 1.22 b | 1.32 b |
Microcistis aeruginosa | 0.94 c | 0.38 c | 4.81 d | 1.62 d | 1.72 d |
Chlorella sp. | 0.87 c | 0.33 c | 4.43 c | 1.44 c | 1.59 c |
Anabaena PCC 7120 | 0.86 c | 0.33 c | 4.41 c | 1.43 c | 1.52 c |
Environmental sample | 0.67 b | 0.25 b | 3.76 b | 1.23 b | 1.35 b |
LSD 0.05 ** | 0.09 | 0.05 | 0.40 | 0.14 | 0.12 |
Applied Preparate Cyanobacteria or Algae | Characteristics of Willow Plant Quality | ||||||
---|---|---|---|---|---|---|---|
Increase in the Length of Shoots % | Content of Macroelements in Plants | Calorific Value in the Operating State kJ kg−1 | Heat of Combustion in the Analytical State kJ kg−1 | The Ash Content in the Working State % | |||
N% | P mg kg d.w−1 | K mg kg d.w−1 | |||||
Control | 100.0 a * | 3.40 a | 2 166 a | 31 910 a | 15 247 a | 18 722 a | 1.60 a |
Bio-Algeen S90 | 110.5 b | 3.48 b | 2 178 ab | 31 920 a | 15 140 a | 18 799 a | 1.59 a |
Microcistis aeruginosa | 125.1 d | 3.64 c | 2 210 c | 31 980 b | 15 223 a | 18 769 a | 1.60 a |
Chlorella sp. | 120.9 c | 3.48 b | 2 201 bc | 31 962 b | 15 224 a | 18 699 a | 1.61 a |
Anabaena PCC 7120 | 118.2 c | 3.58 c | 2 199 bc | 31 960 b | 15 199 a | 18 701 a | 1.62 a |
Environmental sample | 113.7 b | 3.47 ab | 2 176 ab | 31 922 a | 15 187 a | 18 745 a | 1.61 a |
LSD 0.05 ** | 4.0 | 0.7 | 28.9 | 34.3 | 112.1 | 102.2 | 0.3 |
Sample nr. | Mass Reduction, g | Mass Loss, % | Residential Time, min. | Torrefaction Temp., °C |
---|---|---|---|---|
1 | 20/13.17 | 34.15 | 15 | 243.89 |
2 | 20/13.92 | 30.40 | 14 | 244.67 |
3 | 20/13.70 | 31.50 | 16 | 238.99 |
4 | 20/13.97 | 30.15 | 14 | 245.00 |
5 | 20/12.69 | 36.55 | 17 | 241.99 |
6 | 20/12.36 | 38.20 | 20 | 230.02 |
7 | 20/12.03 | 39.85 | 18 | 230.47 |
Energy Crop | Moisture (%) | C ad, (%) | N ad, (%) | H ad, (%) | S ad, (%) | Cl, (%) | Volatile ad (%) | Ash (%) | High Heating Value, (MJ/kg) |
---|---|---|---|---|---|---|---|---|---|
Salix viminalis L. | 7.12 | 45.69 | 0.52 | 6.37 | 0.05 | 0.115 | 91.29 | 7.12 | 19.79 |
Torrefied Salix viminalis L: | |||||||||
(244.67 °C, 14 min) | 4.05 | 52.51 | 0.19 | 5.85 | 0.05 | 0.014 | 73.37 | 3.01 | 23.48 |
(243.89 °C, 15 min) | 3.93 | 53.20 | 0.19 | 5.85 | 0.05 | 0.014 | 72.81 | 3.2 | 24.02 |
(245.0 °C, 14 min) | 3.78 | 54.53 | 0.19 | 5.72 | 0.05 | 0.014 | 72.27 | 3.46 | 25.10 |
Assessed Material | C | O | K | Ca | Mg | Fe | Si | P | S | Cl | Dry Mass |
---|---|---|---|---|---|---|---|---|---|---|---|
[Atomic, %] | [%] | ||||||||||
Ash composition from torrefied Salix viminalis L. (average values) | 23.75 | 45.82 | 22.01 | 2.60 | 0.84 | 0.02 | 0.29 | 3.07 | 0.17 | 1.43 | 100.00 |
Standard deviation, σ | 2.99 | 2.19 | 2.85 | 0.49 | 0.30 | 0.00 | 0.10 | 0.60 | 0.05 | 0.40 | |
Ash composition from untreated Salix viminalis L. (average values) | 33.50 | 33.08 | 21.09 | 2.22 | 0.98 | 0.11 | 0.46 | 1.16 | 0.19 | 3.13 | 100.00 |
Standard deviation, σ | 3.28 | 4.04 | 3.82 | 0.78 | 0.30 | 0.04 | 0.42 | 0.62 | 0.05 | 1.19 |
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Romanowska-Duda, Z.; Szufa, S.; Grzesik, M.; Piotrowski, K.; Janas, R. The Promotive Effect of Cyanobacteria and Chlorella sp. Foliar Biofertilization on Growth and Metabolic Activities of Willow (Salix viminalis L.) Plants as Feedstock Production, Solid Biofuel and Biochar as C Carrier for Fertilizers via Torrefaction Process. Energies 2021, 14, 5262. https://doi.org/10.3390/en14175262
Romanowska-Duda Z, Szufa S, Grzesik M, Piotrowski K, Janas R. The Promotive Effect of Cyanobacteria and Chlorella sp. Foliar Biofertilization on Growth and Metabolic Activities of Willow (Salix viminalis L.) Plants as Feedstock Production, Solid Biofuel and Biochar as C Carrier for Fertilizers via Torrefaction Process. Energies. 2021; 14(17):5262. https://doi.org/10.3390/en14175262
Chicago/Turabian StyleRomanowska-Duda, Zdzislawa, Szymon Szufa, Mieczysław Grzesik, Krzysztof Piotrowski, and Regina Janas. 2021. "The Promotive Effect of Cyanobacteria and Chlorella sp. Foliar Biofertilization on Growth and Metabolic Activities of Willow (Salix viminalis L.) Plants as Feedstock Production, Solid Biofuel and Biochar as C Carrier for Fertilizers via Torrefaction Process" Energies 14, no. 17: 5262. https://doi.org/10.3390/en14175262