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Article

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

by
Hani A. Moubasher
1,
Bassem A. Balbool
2,*,
Yosra A. Helmy
3,
Amnah Mohammed Alsuhaibani
4,
Ahmed A. Atta
5,
Donia H. Sheir
6 and
Ahmed M. Abdel-Azeem
7,*
1
Botany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, Egypt
2
Biotechnology Department, Faculty of Biotechnology, October University for Modern Sciences and Arts, 6th October 12451, Egypt
3
Department of Animal Hygiene, Zoonoses and Animal Ethology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
4
Department of Physical Sport Science, College of Education, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
5
Department of Physics, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
6
Chemistry of Natural and Microbial Products Department, National Research Center, Giza 12622, Egypt
7
Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt
*
Authors to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2022, 19(2), 680; https://doi.org/10.3390/ijerph19020680
Submission received: 10 November 2021 / Revised: 19 December 2021 / Accepted: 24 December 2021 / Published: 7 January 2022
(This article belongs to the Special Issue New Advances in Environmental Microbiology)
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Abstract

Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, cost-effective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.
Keywords: metabolites1; endophytic fungi; asparaginase; induction; purification; antileukemia metabolites1; endophytic fungi; asparaginase; induction; purification; antileukemia

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MDPI and ACS Style

Moubasher, H.A.; Balbool, B.A.; Helmy, Y.A.; Alsuhaibani, A.M.; Atta, A.A.; Sheir, D.H.; Abdel-Azeem, A.M. Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity. Int. J. Environ. Res. Public Health 2022, 19, 680. https://doi.org/10.3390/ijerph19020680

AMA Style

Moubasher HA, Balbool BA, Helmy YA, Alsuhaibani AM, Atta AA, Sheir DH, Abdel-Azeem AM. Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity. International Journal of Environmental Research and Public Health. 2022; 19(2):680. https://doi.org/10.3390/ijerph19020680

Chicago/Turabian Style

Moubasher, Hani A., Bassem A. Balbool, Yosra A. Helmy, Amnah Mohammed Alsuhaibani, Ahmed A. Atta, Donia H. Sheir, and Ahmed M. Abdel-Azeem. 2022. "Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity" International Journal of Environmental Research and Public Health 19, no. 2: 680. https://doi.org/10.3390/ijerph19020680

APA Style

Moubasher, H. A., Balbool, B. A., Helmy, Y. A., Alsuhaibani, A. M., Atta, A. A., Sheir, D. H., & Abdel-Azeem, A. M. (2022). Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity. International Journal of Environmental Research and Public Health, 19(2), 680. https://doi.org/10.3390/ijerph19020680

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