Next Article in Journal
Differences in Outpatient Health Care Utilization 12 Months after COVID-19 Infection by Race/Ethnicity and Community Social Vulnerability
Next Article in Special Issue
Fast, Ungapped Reads Mapping Using Squid
Previous Article in Journal
Boredom Proneness Predicts Self-Assessed Decision Errors in Sports but Is Unrelated to Risk Taking in General
Previous Article in Special Issue
Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJERPH
Volume 19
Issue 6
10.3390/ijerph19063480
ijerph-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes

by
Ewa Sajnaga
1,*,
Marcin Skowronek
1,
Agnieszka Kalwasińska
2,
Waldemar Kazimierczak
1,
Magdalena Lis
1,
Monika Elżbieta Jach
3 and
Adrian Wiater
4
1
Laboratory of Biocontrol, Production, and Application of EPN, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland
2
Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland
3
Department of Molecular Biology, Institute of Biological Sciences, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland
4
Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2022, 19(6), 3480; https://doi.org/10.3390/ijerph19063480
Submission received: 1 February 2022 / Revised: 11 March 2022 / Accepted: 11 March 2022 / Published: 15 March 2022
(This article belongs to the Special Issue New Advances in Environmental Microbiology)
Browse Figures
Review Reports Versions Notes

Abstract

Root-feeding Amphimallon solstitiale larvae and certain other scarab beetles are the main soil-dwelling pests found in Europe, while entomopathogenic nematodes (EPN) have been used as a biocontrol agent against these species. Our study provides the first detailed characterization of the bacterial community of the midgut in wild A. solstitiale larvae, based on the nanopore sequencing of the 16S rRNA gene. In the whole dataset, we detected 2586 different genera and 11,641 species, with only 83 diverse bacterial genera shared by all studied individuals, which may represent members of the core midgut microbiota of A. solstitiale larvae. Subsequently, we compared the midgut microbiota of EPN-resistant and T0 (prior to EPN exposure) individuals, hypothesizing that resistance to this parasitic infection may be linked to the altered gut community. Compared to the control, the resistant insect microbiota demonstrated lower Shannon and Evenness indices and significant differences in the community structure. Our studies confirmed that the gut microbiota alternation is associated with resistant insects; however, there are many processes involved that can affect the bacterial community. Further research on the role of gut microbiota in insect-parasitic nematode interaction may ultimately lead to the improvement of biological control strategies in insect pest management.
Keywords: gut microbiota; entomopathogenic nematodes; pathogen resistance; host–pathogen interactions; Scarabaeidae; nanopore sequencing; metataxonomics; pest biocontrol gut microbiota; entomopathogenic nematodes; pathogen resistance; host–pathogen interactions; Scarabaeidae; nanopore sequencing; metataxonomics; pest biocontrol
Graphical Abstract

Share and Cite

MDPI and ACS Style

Sajnaga, E.; Skowronek, M.; Kalwasińska, A.; Kazimierczak, W.; Lis, M.; Jach, M.E.; Wiater, A. Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes. Int. J. Environ. Res. Public Health 2022, 19, 3480. https://doi.org/10.3390/ijerph19063480

AMA Style

Sajnaga E, Skowronek M, Kalwasińska A, Kazimierczak W, Lis M, Jach ME, Wiater A. Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes. International Journal of Environmental Research and Public Health. 2022; 19(6):3480. https://doi.org/10.3390/ijerph19063480

Chicago/Turabian Style

Sajnaga, Ewa, Marcin Skowronek, Agnieszka Kalwasińska, Waldemar Kazimierczak, Magdalena Lis, Monika Elżbieta Jach, and Adrian Wiater. 2022. "Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes" International Journal of Environmental Research and Public Health 19, no. 6: 3480. https://doi.org/10.3390/ijerph19063480

APA Style

Sajnaga, E., Skowronek, M., Kalwasińska, A., Kazimierczak, W., Lis, M., Jach, M. E., & Wiater, A. (2022). Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes. International Journal of Environmental Research and Public Health, 19(6), 3480. https://doi.org/10.3390/ijerph19063480

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop