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Marine Drugs
Volume 22
Issue 11
10.3390/md22110521
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Article

Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster

by
Shu-Mei Shen
1,†,
Yun-Chang Xie
2,†,
Li-Rong Tu
1,
Miao-Er Wu
2,
Yan-Min Wang
2,
Chun-Hui Song
2,
Yu-Hui Sun
3,* and
Ming-He Luo
1,*
1
School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
2
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
3
School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Mar. Drugs 2024, 22(11), 521; https://doi.org/10.3390/md22110521
Submission received: 30 October 2024 / Revised: 15 November 2024 / Accepted: 18 November 2024 / Published: 20 November 2024
(This article belongs to the Special Issue Marine Streptomyces-Derived Natural Products 2024)
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Abstract

Chemically investigating the marine-derived Streptomyces parvus 1268 led to the isolation of a new compound of carpatamide I (1). Subsequent genomic analysis identified its candidate biosynthetic gene cluster ctd of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J–M (2–5). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds 1–5 displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.
Keywords: carpatamides; manumycin; natural products; genome mining carpatamides; manumycin; natural products; genome mining
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MDPI and ACS Style

Shen, S.-M.; Xie, Y.-C.; Tu, L.-R.; Wu, M.-E.; Wang, Y.-M.; Song, C.-H.; Sun, Y.-H.; Luo, M.-H. Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster. Mar. Drugs 2024, 22, 521. https://doi.org/10.3390/md22110521

AMA Style

Shen S-M, Xie Y-C, Tu L-R, Wu M-E, Wang Y-M, Song C-H, Sun Y-H, Luo M-H. Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster. Marine Drugs. 2024; 22(11):521. https://doi.org/10.3390/md22110521

Chicago/Turabian Style

Shen, Shu-Mei, Yun-Chang Xie, Li-Rong Tu, Miao-Er Wu, Yan-Min Wang, Chun-Hui Song, Yu-Hui Sun, and Ming-He Luo. 2024. "Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster" Marine Drugs 22, no. 11: 521. https://doi.org/10.3390/md22110521

APA Style

Shen, S.-M., Xie, Y.-C., Tu, L.-R., Wu, M.-E., Wang, Y.-M., Song, C.-H., Sun, Y.-H., & Luo, M.-H. (2024). Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster. Marine Drugs, 22(11), 521. https://doi.org/10.3390/md22110521

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