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Anticoagulant Activity of Sulfated Ulvan Isolated from the Green Macroalga Ulva rigida

Equipe BCBS (Biotechnologies et Chimie des Bioressources pour la Santé), La Rochelle Université, UMR CNRS 7266 LIENSs, Avenue Michel Crépeau, 17042 La Rochelle, France
SEPROSYS, Séparations, Procédés, Systèmes, 12 Rue Marie-Aline Dusseau, 17000 La Rochelle, France
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(5), 291;
Received: 2 April 2019 / Revised: 30 April 2019 / Accepted: 7 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Characterization of Bioactive Components in Edible Algae)
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(1) Background: Brown and red algal sulfated polysaccharides have been widely described as anticoagulant agents. However, data on green algae, especially on the Ulva genus, are limited. This study aimed at isolating ulvan from the green macroalga Ulva rigida using an acid- and solvent-free procedure, and investigating the effect of sulfate content on the anticoagulant activity of this polysaccharide. (2) Methods: The obtained ulvan fraction was chemically sulfated, leading to a doubling of the polysaccharide sulfate content in a second ulvan fraction. The potential anticoagulant activity of both ulvan fractions was then assessed using different assays, targeting the intrinsic and/or common (activated partial thromboplastin time), extrinsic (prothrombin time), and common (thrombin time) pathways, and the specific antithrombin-dependent pathway (anti-Xa and anti-IIa), of the coagulation cascade. Furthermore, their anticoagulant properties were compared to those of commercial anticoagulants: heparin and Lovenox®. (3) Results: The anticoagulant activity of the chemically-sulfated ulvan fraction was stronger than that of Lovenox® against both the intrinsic and extrinsic coagulation pathways. (4) Conclusion: The chemically-sulfated ulvan fraction could be a very interesting alternative to heparins, with different targets and a high anticoagulant activity. View Full-Text
Keywords: Ulva rigida; ulvan; chemical sulfation; anticoagulant activity Ulva rigida; ulvan; chemical sulfation; anticoagulant activity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Adrien, A.; Bonnet, A.; Dufour, D.; Baudouin, S.; Maugard, T.; Bridiau, N. Anticoagulant Activity of Sulfated Ulvan Isolated from the Green Macroalga Ulva rigida. Mar. Drugs 2019, 17, 291.

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