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Mar. Drugs 2019, 17(4), 209; https://doi.org/10.3390/md17040209

Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization

1
CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
2
Marine and Environmental Sciences Centre (MARE), Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal
3
CESAM–Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
4
QOPNA–Organic Chemistry, Natural Products and Food Stuffs Research Unit & Department of Chemistry, University of Aveiro, Aveiro, 3810-193, Portugal
*
Author to whom correspondence should be addressed.
Received: 27 February 2019 / Revised: 27 March 2019 / Accepted: 29 March 2019 / Published: 3 April 2019
(This article belongs to the Special Issue Characterization of Bioactive Components in Edible Algae)
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Abstract

Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2–27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4–2.7 g/100 gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes. View Full-Text
Keywords: Osmundea pinnatifida; Sargassum muticum; enzymatic extracts; minerals; mono and polysaccharides; FTIR-ATR; NMR; cytotoxicity Osmundea pinnatifida; Sargassum muticum; enzymatic extracts; minerals; mono and polysaccharides; FTIR-ATR; NMR; cytotoxicity
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Rodrigues, D.; Costa-Pinto, A.R.; Sousa, S.; Vasconcelos, M.W.; Pintado, M.M.; Pereira, L.; Rocha-Santos, T.A.; Costa, J.P.; Silva, A.M.; Duarte, A.C.; Gomes, A.M.; Freitas, A.C. Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization. Mar. Drugs 2019, 17, 209.

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