Four Antioxidant Peptides from Protein Hydrolysate of Red Stingray (Dasyatis akajei) Cartilages: Isolation, Identification, and In Vitro Activity Evaluation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation of APs from Water-Soluble Protein Hydrolysate (RSH) of Red Stingray Cartilages
2.1.1. Fractionation of RSH by Ultrafiltration
2.1.2. Anion-Exchange Chromatography of RSH-I
2.1.3. Gel filtration Chromatography of RSH-I-4
2.1.4. Isolation of APs from Frac.1 by RP-HPLC
2.2. Amino Acid Sequence and Mass Spectrometry Analysis of APs (RSHP-A~D)
2.3. Antioxidant Activity
2.3.1. Radical Scavenging Activity
DPPH• Scavenging Activity
HO• Scavenging Activity
• Scavenging Activity
ABTS+• Scavenging Activity
2.3.2. Fe2+-Chelating Ability
2.3.3. Reducing Power
2.3.4. Lipid Peroxidation Inhibition Ability
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Preparation of Water-Soluble Proteins and Hydrolysate from Red Stingray Cartilages
4.3. Isolation of Peptides from RSH
4.4. Amino Acid Sequence and Molecular Mass Analysis
4.5. Antioxidant Activity
4.5.1. Radical Scavenging Activity
4.5.2. Fe2+-Chelating Assay
4.5.3. Reducing Power Assay
4.5.4. Lipid Peroxidation Inhibition Assay
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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RSHP-A | RSHP-B | RSHP-C | RSHP-D | ||
---|---|---|---|---|---|
Retention time (min) | 9.434 | 13.435 | 14.137 | 18.610 | |
Amino acid sequence | VPR | IEPH | LEEEE | IEEEQ | |
Theoretical mass/ observed mass (Da) | 370.44/ 370.46 | 494.52/ 494.55 | 647.69/ 647.64 | 646.62/ 646.66 | |
EC50 (mg/mL) | DPPH• | 4.61 | 0.77 | 0.08 | 0.15 |
HO• | 1.90 | 0.46 | 0.17 | 0.11 | |
• | 3.69 | 0.70 | 0.15 | 0.19 | |
ABTS+• | 4.01 | 1.30 | 0.16 | 0.18 |
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Pan, X.-Y.; Wang, Y.-M.; Li, L.; Chi, C.-F.; Wang, B. Four Antioxidant Peptides from Protein Hydrolysate of Red Stingray (Dasyatis akajei) Cartilages: Isolation, Identification, and In Vitro Activity Evaluation. Mar. Drugs 2019, 17, 263. https://doi.org/10.3390/md17050263
Pan X-Y, Wang Y-M, Li L, Chi C-F, Wang B. Four Antioxidant Peptides from Protein Hydrolysate of Red Stingray (Dasyatis akajei) Cartilages: Isolation, Identification, and In Vitro Activity Evaluation. Marine Drugs. 2019; 17(5):263. https://doi.org/10.3390/md17050263
Chicago/Turabian StylePan, Xiao-Yang, Yu-Mei Wang, Li Li, Chang-Feng Chi, and Bin Wang. 2019. "Four Antioxidant Peptides from Protein Hydrolysate of Red Stingray (Dasyatis akajei) Cartilages: Isolation, Identification, and In Vitro Activity Evaluation" Marine Drugs 17, no. 5: 263. https://doi.org/10.3390/md17050263