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Collagen Extracted from Bigeye Tuna (Thunnus obesus) Skin by Isoelectric Precipitation: Physicochemical Properties, Proliferation, and Migration Activities

1
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
2
Zhoushan Institute for Food and Drug Control, Zhoushan 316000, China
3
Zhejiang Ocean Family CO., LTD, Zhoushan 316022, China
4
Zhejiang Changxing Pharmaceutical CO., LTD, Huzhou 313108, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2019, 17(5), 261; https://doi.org/10.3390/md17050261
Received: 22 April 2019 / Revised: 28 April 2019 / Accepted: 30 April 2019 / Published: 1 May 2019
Collagen was extracted from bigeye tuna (Thunnus obesus) skins by salting-out (PSC-SO) and isoelectric precipitation (PSC-IP) methods. The yield of the PSC-IP product was approximately 17.17% (dry weight), which was greater than the yield obtained from PSC-SO (14.14% dry weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that collagen from bigeye tuna skin belongs to collagen type I. Inductively coupled plasma mass spectrometry results indicate that the heavy metal abundance in PSC-IP was lower than the maximum acceptable amounts according to Chinese regulatory standards. In addition, results from a methylthiazolyldiphenyl-tetrazolium bromide assay and an in vitro scratch assay demonstrated that PSC-IP could promote the proliferation and migration of NIH-3T3 fibroblasts. Overall, results suggest PSC-IP could be used to rapidly extract collagen from marine by-products instead of traditional salting-out methods. Collagen from bigeye tuna skin may also have strong potential for cosmetic and biomedical applications. View Full-Text
Keywords: Thunnus obesus; collagen; isoelectric precipitation; physicochemical properties; proliferation and migration Thunnus obesus; collagen; isoelectric precipitation; physicochemical properties; proliferation and migration
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Lin, X.; Chen, Y.; Jin, H.; Zhao, Q.; Liu, C.; Li, R.; Yu, F.; Chen, Y.; Huang, F.; Yang, Z.; Ding, G.; Tang, Y. Collagen Extracted from Bigeye Tuna (Thunnus obesus) Skin by Isoelectric Precipitation: Physicochemical Properties, Proliferation, and Migration Activities. Mar. Drugs 2019, 17, 261.

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