16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. DNA Extraction
2.3. 16S rRNA Sequencing
2.4. Bioinformatics Analysis
3. Results
3.1. Baseline Characteristics
3.2. Results of Ascites 16S rRNA Gene Sequencing Test
3.3. Clinical Course of the Patients
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Sex | Age (Years) | Etiology | Ascites Culture | Ascites Color | Ascites Turbidity | Ascites WBC (Cells/µL) | Ascites PMN (%) | SAAG (g/dL) | Ascites Protein (mg/dL) |
---|---|---|---|---|---|---|---|---|---|---|
1 | F | 78 | Ovarian cancer | Absence of bacterial growth | Yellow | Clear | 135 | 72 | 0.9 | 1270 |
2 | F | 69 | Ovarian cancer | Absence of bacterial growth | Yellow | Cloudy | 1602 | 16 | 0.4 | 5291 |
3 | F | 64 | Colon cancer with liver metastasis | Absence of bacterial growth | Yellow | Clear | 198 | 6 | 1.7 | 775 |
4 | M | 59 | HCV-LC | Absence of bacterial growth | Red | Cloudy | 154 | 100 | 2.1 | 1027 |
5 | F | 89 | Biliary cirrhosis | Absence of bacterial growth | Yellow | Clear | 210 | 65 | 1.7 | 1254 |
6 | M | 62 | HBV-LC | Absence of bacterial growth | Yellow | Clear | 77 | 49 | 2.1 | 1151 |
7 | M | 64 | NBNC-LC | Absence of bacterial growth | Dark yellow | Cloudy | 108 | 4 | 1.8 | 1163 |
8 | M | 63 | NBNC-LC | Absence of bacterial growth | Yellow | Clear | 42 | 2 | 2.3 | 568 |
9 | M | 52 | NBNC-LC | Absence of bacterial growth | Yellow | Clear | 42 | 1 | 2.7 | 892 |
10 | M | 79 | NBNC-LC | Absence of bacterial growth | Yellow | Clear | 144 | 26 | 1.7 | 775 |
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Yang, C.J.; Song, J.S.; Yoo, J.-J.; Park, K.W.; Yun, J.; Kim, S.G.; Kim, Y.S. 16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis. Medicina 2024, 60, 289. https://doi.org/10.3390/medicina60020289
Yang CJ, Song JS, Yoo J-J, Park KW, Yun J, Kim SG, Kim YS. 16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis. Medicina. 2024; 60(2):289. https://doi.org/10.3390/medicina60020289
Chicago/Turabian StyleYang, Chan Jin, Ju Sun Song, Jeong-Ju Yoo, Keun Woo Park, Jina Yun, Sang Gyune Kim, and Young Seok Kim. 2024. "16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis" Medicina 60, no. 2: 289. https://doi.org/10.3390/medicina60020289