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Article

Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins

1
Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan
2
Laboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
3
Center for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan
4
Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan
5
Institute for Protein Research, Osaka University, Osaka 565-0871, Japan
*
Author to whom correspondence should be addressed.
These authors equally contributed as first authors.
Academic Editor: Rinaldo Pellicano
Medicina 2021, 57(3), 275; https://doi.org/10.3390/medicina57030275
Received: 2 February 2021 / Revised: 12 March 2021 / Accepted: 12 March 2021 / Published: 16 March 2021
(This article belongs to the Special Issue A Focus on Twin Studies)
Background and Objectives: The gut microbiota is associated with human health and dietary nutrition. Various studies have been reported in this regard, but it is difficult to clearly analyze human gut microbiota as individual differences are significant. The causes of these individual differences in intestinal microflora are genetic and/or environmental. In this study, we focused on differences between identical twins in Japan to clarify the effects of nutrients consumed on the entire gut microbiome, while excluding genetic differences. Materials and Methods: We selected healthy Japanese monozygotic twins for the study and confirmed their zygosity by matching 15 short tandem repeat loci. Their fecal samples were subjected to 16S rRNA sequencing and bioinformatics analyses to identify and compare the fluctuations in intestinal bacteria. Results: We identified 12 genera sensitive to environmental factors, and found that Lactobacillus was relatively unaffected by environmental factors. Moreover, we identified protein, fat, and some nutrient intake that can affect 12 genera, which have been identified to be more sensitive to environmental factors. Among the 12 genera, Bacteroides had a positive correlation with retinol equivalent intake (rs = 0.38), Lachnospira had a significantly negative correlation with protein, sodium, iron, vitamin D, vitamin B6, and vitamin B12 intake (rs = −0.38, −0.41, −0.39, −0.63, −0.42, −0.49, respectively), Lachnospiraceae ND3007 group had a positive correlation with fat intake (rs = 0.39), and Lachnospiraceae UCG-008 group had a negative correlation with the saturated fatty acid intake (rs = −0.45). Conclusions: Our study is the first to focus on the relationship between human gut microbiota and nutrient intake using samples from Japanese twins to exclude the effects of genetic factors. These findings will broaden our understanding of the more intuitive relationship between nutrient intake and the gut microbiota and can be a useful basis for finding useful biomarkers that contribute to human health. View Full-Text
Keywords: monozygotic twins; gut microbiome; nutrients monozygotic twins; gut microbiome; nutrients
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MDPI and ACS Style

Matsumoto, N.; Park, J.; Tomizawa, R.; Kawashima, H.; Hosomi, K.; Mizuguchi, K.; Honda, C.; Ozaki, R.; Iwatani, Y.; Watanabe, M.; Kunisawa, J. Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins. Medicina 2021, 57, 275. https://doi.org/10.3390/medicina57030275

AMA Style

Matsumoto N, Park J, Tomizawa R, Kawashima H, Hosomi K, Mizuguchi K, Honda C, Ozaki R, Iwatani Y, Watanabe M, Kunisawa J. Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins. Medicina. 2021; 57(3):275. https://doi.org/10.3390/medicina57030275

Chicago/Turabian Style

Matsumoto, Natsuko, Jonguk Park, Rie Tomizawa, Hitoshi Kawashima, Koji Hosomi, Kenji Mizuguchi, Chika Honda, Ritsuko Ozaki, Yoshinori Iwatani, Mikio Watanabe, and Jun Kunisawa. 2021. "Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins" Medicina 57, no. 3: 275. https://doi.org/10.3390/medicina57030275

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