Developmental Cues and Molecular Drivers in Myelinogenesis: Revisiting Early Life to Re-Evaluate the Integrity of CNS Myelin

Round 1

Reviewer 1 Report

In this manuscript Dermitzakis and colleagues describe myelinogenesis and myelin development from early stages until maturation. This review is very well-written and the figures are particularly meaningful.

Please, always specify throughout the manuscript when results have been obtained in humans. All the others are supposed to be from mouse.

Minor points

Lane 37, latter instead of later

Lane 37, are you sure “uniformely” is the proper adverb?

Lane 91, Is it E12.5?

Lane 93, delete an

Author Response

We would like to thank the reviewer for the fruitful comments. All suggestions and minor points have been addressed and changes are now reflected in the revised manuscript (track changes enabled).

All in all, we very much appreciate the encouraging, critical and constructive comments on this manuscript by the Reviewer 1. The comments have been very thorough and useful in improving the manuscript.

Reviewer 2 Report

The manuscript entitled « Developmental cues and molecular drivers in myelinogenesis - revisiting early life to re-evaluate the integrity of myelin » is particularly well organized, documented and enjoyable to read. It provides an interesting overview of the oligodendrocyte lineage development, with an exhaustive list of molecular cues, highlighting the most prominent ones  -
Few transcription factors function specifically at the postmitotic stage of the OL lineage, and the role of Mrf which is only briefly mentioned line 237 could be more highlighted, as it is a critical transcriptional regulator essential for oligodendrocyte maturation and CNS myelination. Whereas the majority of transcription factors within the OL lineage are critical for the specification of OPCs or initial differentiation of OLs, MRF is unique in coordinating subsequent maturation/myelination of postmitotic OLs (B. Emery et al, Cell, 138, 2009).
I have another comment, line 333, « a somatodendritic protein JAM2 is further localised in OLs » - Please modify as JAM2 expression is only neuronal (according to the ref cited).
Some typo errors line 93 suppress an, line 403 cues instead of ques?

Author Response

We highly appreciate the encouraging comments of the reviewer. All critical recommendations have been addressed and the revised manuscript now reflects all additional suggested changes that can be seen through the track changes function.

All in all, we thank the Reviewer 2 for the critical and diligent scrutiny of the submitted manuscript.