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Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro

Department of Animal Biotechnology and Molecular Diagnostics, L.K. Ernst Federal Research Center for Animal Husbandry, 142132 Podolsk, Russia
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Author to whom correspondence should be addressed.
Academic Editors: Alexander V. Sirotkin and Adriana Kolesarova
Pharmaceuticals 2021, 14(7), 684; https://doi.org/10.3390/ph14070684
Received: 9 June 2021 / Revised: 5 July 2021 / Accepted: 13 July 2021 / Published: 16 July 2021
(This article belongs to the Special Issue Novel Regulators of Female Reproduction)
Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study the patterns and intracellular pathways of actions of prolactin (PRL) and growth hormone (GH) on age-associated changes in bovine M-II oocytes aging in vitro. To this end, we analyzed spontaneous parthenogenetic activation (cytogenetic assay), apoptosis (TUNEL assay), and the developmental capacity (IVF/IVC) of in vitro-matured oocytes after prolonged culturing. Both PRL and GH reduced the activation rate of aging cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs), and their respective hormone receptors were revealed in the ova. The inhibitor of Src-family tyrosine kinases PP2 eliminated the effects of PRL and GH on meiotic arrest in DOs, whereas the MEK inhibitor U0126 only abolished the PRL effect. Furthermore, PRL was able to maintain the apoptosis resistance and developmental competence of aging CEOs. The protein kinase C inhibitor calphostin C suppressed both the actions of PRL. Thus, PRL and GH can directly support meiotic arrest in aging M-II oocytes by activating MAP kinases and/or Src-family kinases. The effect of PRL in maintaining the developmental capacity of aging oocytes is cumulus-dependent and related to the pro-survival action of the protein kinase C-mediated signal pathway. View Full-Text
Keywords: prolactin; growth hormone; oocyte aging; spontaneous parthenogenetic activation; apoptosis; developmental capacity; receptors; cumulus cells; signaling pathways prolactin; growth hormone; oocyte aging; spontaneous parthenogenetic activation; apoptosis; developmental capacity; receptors; cumulus cells; signaling pathways
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MDPI and ACS Style

Singina, G.N.; Shedova, E.N.; Lopukhov, A.V.; Mityashova, O.S.; Lebedeva, I.Y. Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro. Pharmaceuticals 2021, 14, 684. https://doi.org/10.3390/ph14070684

AMA Style

Singina GN, Shedova EN, Lopukhov AV, Mityashova OS, Lebedeva IY. Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro. Pharmaceuticals. 2021; 14(7):684. https://doi.org/10.3390/ph14070684

Chicago/Turabian Style

Singina, Galina N., Ekaterina N. Shedova, Alexander V. Lopukhov, Olga S. Mityashova, and Irina Y. Lebedeva 2021. "Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro" Pharmaceuticals 14, no. 7: 684. https://doi.org/10.3390/ph14070684

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