Next Article in Journal
Thermographic Phosphors for High Temperature Measurements: Principles, Current State of the Art and Recent Applications
Previous Article in Journal
Electrochemical Behaviors of Methylene Blue on DNA Modified Electrode and Its Application to the Detection of PCR Product from NOS Sequence
Open AccessArticle

An Immunosensing System Using Stilbene Glycoside as a Fluorogenic Substrate for an Enzymatic Reaction Model

College of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410076, Hunan, P.R. China
*
Author to whom correspondence should be addressed.
Sensors 2008, 8(9), 5661-5672; https://doi.org/10.3390/s8095661
Received: 21 July 2008 / Revised: 4 September 2008 / Accepted: 11 September 2008 / Published: 15 September 2008
A natural product, stilbene glycoside (2,3,5,4’-tetrahydroxydiphenylethylene-2-O-glucoside, TBG), has been evaluated for the first time as a potential substrate for horseradish peroxidase (HRP)-catalyzed fluorogenic reactions. The properties of TBG as a fluorogenic substrate for HRP and its application in a fluorometric enzyme-linked immunosensing system were compared with commercially available substrates such as p-hydroxyphenylpropionic acid (pHPPA), chavicol and Amplex red using Brucella melitensis antibody (BrAb) as a model analyte. The immunosensing body based on HRP-BrAb was constructed by dispersing graphite, BrAg and paraffin wax at room temperature. In a competitive immunoassay procedure, the BrAb competed with HRP-BrAb to react with the immobilized BrAg. In the enzymatic reaction, the binding HRP-BrAb on the sensing body surface can catalyze the polymerization reaction of TBG by H2O2 forming fluorescent dimers and causing an increase in fluorescence intensity. TBG showed comparable ability for HRP detection and its enzyme-linked immunosensing reaction system, in a linear detection ranging of 3.5´10-8~7.6´10-6g/L and with a detection limit of 1.7´10-9 g/L. The immobilized biocomposite surface could be regenerated with excellent reproducibility (RSD=3.8%) by simply polishing with an alumina paper. The proposed immunosensing system has been used to determine the BrAb in rabbit serum samples with satisfactory results. View Full-Text
Keywords: Stilbene glycoside; HRP fluorogenic substrate; enzyme-linked immunosensing; Brucella melitensis Stilbene glycoside; HRP fluorogenic substrate; enzyme-linked immunosensing; Brucella melitensis
MDPI and ACS Style

Zhan, X.-H.; Gong, F.-C.; Tan, S.-Z.; Huang, P.-M.; Tan, Y.-F. An Immunosensing System Using Stilbene Glycoside as a Fluorogenic Substrate for an Enzymatic Reaction Model. Sensors 2008, 8, 5661-5672.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop