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Article

Evaluation of Low-Cost Multi-Spectral Sensors for Measuring Chlorophyll Levels Across Diverse Leaf Types

by
Prattana Lopin
1,2,
Pichapob Nawsang
1,
Srisangwan Laywisadkul
1 and
Kyle V. Lopin
3,*
1
Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
2
Center of Excellence for Biodiversity, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
3
Department of Physics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
*
Author to whom correspondence should be addressed.
Sensors 2025, 25(7), 2198; https://doi.org/10.3390/s25072198
Submission received: 28 January 2025 / Revised: 15 March 2025 / Accepted: 26 March 2025 / Published: 31 March 2025
(This article belongs to the Section Biosensors)

Abstract

Chlorophyll levels are a key indicator of plant nitrogen status, which plays a critical role in optimizing agricultural yields. This study evaluated the performance of three low-cost multi-spectral sensors, AS7262, AS7263, and AS7265x, for non-destructive chlorophyll measurement. Measurements were taken from a diverse set of five leaf types, including smooth, uniform leaves (banana and mango), textured leaves (jasmine and sugarcane), and narrow leaves (rice). Partial least squares regression models were used to fit sensor spectra to chlorophyll levels, using nested cross-validation to ensure robust model evaluation. Sensor performance was assessed using R2 and mean absolute error (MAE) scores. The AS7265x demonstrated the best performance on smooth, uniform leaves with validation R2 scores of 0.96–0.95. Its performance decreased for the other leaves, with R2 scores of 0.75–0.85. The AS7262 and AS7263 sensors, while slightly less accurate, achieved reasonable R2 scores ranging from 0.93 to 0.86 for smooth leaves, and from 0.85 to 0.73 for the other leaves. All sensors, particularly the AS7265x, show potential for non-destructive chlorophyll measurement in agricultural applications. Their low cost and reasonable accuracy make them suitable for agricultural applications such as monitoring plant nitrogen levels.
Keywords: chlorophyll sensors; low-cost sensors; spectral sensors; spectral reflectance; partial least squares regression; nested cross-validation; learning curves; model validation; machine learning chlorophyll sensors; low-cost sensors; spectral sensors; spectral reflectance; partial least squares regression; nested cross-validation; learning curves; model validation; machine learning

Share and Cite

MDPI and ACS Style

Lopin, P.; Nawsang, P.; Laywisadkul, S.; Lopin, K.V. Evaluation of Low-Cost Multi-Spectral Sensors for Measuring Chlorophyll Levels Across Diverse Leaf Types. Sensors 2025, 25, 2198. https://doi.org/10.3390/s25072198

AMA Style

Lopin P, Nawsang P, Laywisadkul S, Lopin KV. Evaluation of Low-Cost Multi-Spectral Sensors for Measuring Chlorophyll Levels Across Diverse Leaf Types. Sensors. 2025; 25(7):2198. https://doi.org/10.3390/s25072198

Chicago/Turabian Style

Lopin, Prattana, Pichapob Nawsang, Srisangwan Laywisadkul, and Kyle V. Lopin. 2025. "Evaluation of Low-Cost Multi-Spectral Sensors for Measuring Chlorophyll Levels Across Diverse Leaf Types" Sensors 25, no. 7: 2198. https://doi.org/10.3390/s25072198

APA Style

Lopin, P., Nawsang, P., Laywisadkul, S., & Lopin, K. V. (2025). Evaluation of Low-Cost Multi-Spectral Sensors for Measuring Chlorophyll Levels Across Diverse Leaf Types. Sensors, 25(7), 2198. https://doi.org/10.3390/s25072198

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