A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells
by
Yueyuan Mao 1,2,*, Yediao Xu 1, Zhi Li 1, Yang Wang 1, Huanhuan Du 1, Lei Liu 1, Ran Ding 1 and Guodong Liu 3,*
Yueyuan Mao 1,2,*, Yediao Xu 1, Zhi Li 1, Yang Wang 1, Huanhuan Du 1, Lei Liu 1, Ran Ding 1 and Guodong Liu 3,*
1
College of Chemistry and Materials Engineering, Anhui Science and Technology University, Bengbu 233030, China
2
State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China
3
Institute of Biomedical and Health Science, Anhui Science and Technology University, Bengbu 233030, China
*
Authors to whom correspondence should be addressed.
Sensors 2019, 19(24), 5348; https://doi.org/10.3390/s19245348
Received: 15 November 2019 / Revised: 1 December 2019 / Accepted: 2 December 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Luminescent/Colorimetric Probes and Sensors)
Intracellular GSH is the most abundant non-protein biothiol and acts as a central antioxidant to defend against aging toxins and radicals. Meanwhile abnormal level of intracellular GSH concentration is directly related to some diseases. In this case, detecting intracellular GSH rapidly and sensitively is of great significance. We synthesize a simple fluorescent probe (named GP) which can discriminate GSH from Cys (cysteine) or Hcy (homocysteine) and presents a 50-fold fluorescence increasing. The response time of GP to GSH was only 5 min and the product GO (the product of GP after reacting with GSH) after reacting with GSH possesses a larger Stokes shift for 135 nm than that in reported work. Probe GP can detect intracellular effectively and shows obvious yellow fluorescence. Briefly, probe GP can detect intracellular GSH rapidly and effectively both in vitro and in living cells.
Keywords:
fluorescent probe; GSH; cell imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Mao, Y.; Xu, Y.; Li, Z.; Wang, Y.; Du, H.; Liu, L.; Ding, R.; Liu, G. A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells. Sensors 2019, 19, 5348.
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