Next Article in Journal
Electronic Tongue Coupled to an Electrochemical Flow Reactor for Emerging Organic Contaminants Real Time Monitoring
Previous Article in Journal
Linear-Time Direct Data Assignment Algorithm for Passive Sensor Measurements
Previous Article in Special Issue
A Quantum Dot-Based FLIM Glucose Nanosensor
Open AccessArticle

A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells

College of Chemistry and Materials Engineering, Anhui Science and Technology University, Bengbu 233030, China
State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China
Institute of Biomedical and Health Science, Anhui Science and Technology University, Bengbu 233030, China
Authors to whom correspondence should be addressed.
Sensors 2019, 19(24), 5348;
Received: 15 November 2019 / Revised: 1 December 2019 / Accepted: 2 December 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Luminescent/Colorimetric Probes and Sensors)
Intracellular GSH is the most abundant non-protein biothiol and acts as a central antioxidant to defend against aging toxins and radicals. Meanwhile abnormal level of intracellular GSH concentration is directly related to some diseases. In this case, detecting intracellular GSH rapidly and sensitively is of great significance. We synthesize a simple fluorescent probe (named GP) which can discriminate GSH from Cys (cysteine) or Hcy (homocysteine) and presents a 50-fold fluorescence increasing. The response time of GP to GSH was only 5 min and the product GO (the product of GP after reacting with GSH) after reacting with GSH possesses a larger Stokes shift for 135 nm than that in reported work. Probe GP can detect intracellular effectively and shows obvious yellow fluorescence. Briefly, probe GP can detect intracellular GSH rapidly and effectively both in vitro and in living cells.
Keywords: fluorescent probe; GSH; cell imaging fluorescent probe; GSH; cell imaging
Show Figures

Graphical abstract

MDPI and ACS Style

Mao, Y.; Xu, Y.; Li, Z.; Wang, Y.; Du, H.; Liu, L.; Ding, R.; Liu, G. A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells. Sensors 2019, 19, 5348.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop