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Sensors 2019, 19(1), 66; https://doi.org/10.3390/s19010066

Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method

1
Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Polígono del Río San Pedro, S/N. 11510 Puerto Real, 11001 Cadiz, Spain
2
Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania
3
Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
Received: 29 November 2018 / Revised: 14 December 2018 / Accepted: 19 December 2018 / Published: 25 December 2018
(This article belongs to the Special Issue Nanostructured Surfaces in Sensing Systems)
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Abstract

The application of a novel Poly(3,4-ethylenedioxythiophene)-Tyrosinase/Sonogel-Carbon electrode (PEDOT-Tyr/SNGC) biosensor to beers and wines analysis is proposed. This biosensor implies a new Sinusoidal Current (SC) electrodeposition method to immobilize the enzyme generating a nanostructure surface. The biosensors were characterized electrochemically, employing cyclic voltammetry and electrochemical impedance spectroscopy. Sensitivity, limit of detection, and correlation coefficients of the linear fitting were 2.40 × 10−4 µA·µM−1, 4.33 µM, and R2 = 0.9987, respectively. Caffeic acid is used as the reference polyphenol. A sampling of nine beers (four lager, three stout, and two non-alcoholic beers), and four wines (three red and one white wine) purchased in a local store was performed. The Polyphenol indeces for beers and wines have been assessed using the proposed biosensor, and the obtained values are in agreement with the literature data. Antioxidant properties of the samples using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical spectrophotometric method were also evaluated. The correlation between the polyphenol index and the antioxidant capacity was obtained for beers and wines. View Full-Text
Keywords: sonogel–carbon based biosensor; sinusoidal current method; polyphenol index; antioxidant capacity; beer and wine sonogel–carbon based biosensor; sinusoidal current method; polyphenol index; antioxidant capacity; beer and wine
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García-Guzmán, J.J.; López-Iglesias, D.; Cubillana-Aguilera, L.; Lete, C.; Lupu, S.; Palacios-Santander, J.M.; Bellido-Milla, D. Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method. Sensors 2019, 19, 66.

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