A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection
AbstractDigital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities. Here, we demonstrate for the first time the role of coupling DMF and LAMP, in a dedicated device that allows straightforward mixing of LAMP reagents and target DNA, as well as optimum temperature control (reaction droplets undergo a temperature variation of just 0.3 °C, for 65 °C at the bottom plate). This device is produced using low-temperature and low-cost production processes, adaptable to disposable and flexible substrates. DMF-LAMP is performed with enhanced sensitivity without compromising reaction efficacy or losing reliability and efficiency, by LAMP-amplifying 0.5 ng/µL of target DNA in just 45 min. Moreover, on-chip LAMP was performed in 1.5 µL, a considerably lower volume than standard bench-top reactions. View Full-Text
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Coelho, B.J.; Veigas, B.; Águas, H.; Fortunato, E.; Martins, R.; Baptista, P.V.; Igreja, R. A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection. Sensors 2017, 17, 2616.
Coelho BJ, Veigas B, Águas H, Fortunato E, Martins R, Baptista PV, Igreja R. A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection. Sensors. 2017; 17(11):2616.Chicago/Turabian Style
Coelho, Beatriz J.; Veigas, Bruno; Águas, Hugo; Fortunato, Elvira; Martins, Rodrigo; Baptista, Pedro V.; Igreja, Rui. 2017. "A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection." Sensors 17, no. 11: 2616.
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