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Open AccessArticle

Nitrogen Fixing and Phosphate Mineralizing Bacterial Communities in Sweet Potato Rhizosphere Show a Genotype-Dependent Distribution

1
Instituto de Ciências Biológicas, Centro de Genômica e Biologia de Sistemas, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, CEP 66.075-110, Belém, PA, Brazil
2
Instituto de Microbiologia Paulo de Góes (IMPPG), Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brazil
3
Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, Av. Marechal Rondon S/N, CEP 49100-000, São Cristóvão, SE, Brazil
*
Author to whom correspondence should be addressed.
Diversity 2019, 11(12), 231; https://doi.org/10.3390/d11120231
Received: 23 October 2019 / Revised: 20 November 2019 / Accepted: 28 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Genetic Diversity of Soil Bacterial Communities)
We hypothesize that sweet potato genotypes can influence the bacterial communities related to phosphate mineralization and nitrogen fixation in the rhizosphere. Tuberous roots of field-grown sweet potato from genotypes IPB-149, IPB-052, and IPB-137 were sampled three and six months after planting. The total community DNA was extracted from the rhizosphere and analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and quantitative real-time PCR (qPCR), based on the alkaline phosphatase coding gene (alp gene) and on the nitrogenase coding gene (nifH gene). The cluster analysis based on DGGE showed that plant age slightly influenced the bacterial community related to phosphate mineralization in the rhizosphere of IPB-137, although it did not affect the bacterial community related to nitrogen fixation. The statistical analysis of DGGE fingerprints (Permutation test, p ≤ 0.05) showed that nitrogen-fixing bacterial community of IPB-052 statistically differed from genotypes IPB-149 and IPB-137 after six months of planting. The bacterial community of IPB-137 rhizosphere analyzed by alp gene also showed significant differences when compared to IPB-149 in both sampling times (p ≤ 0.05). In addition, alp gene copy numbers significantly increased in abundance in the rhizosphere of IPB-137 after six months of planting. Therefore, plant genotype should be considered in the biofertilization of sweet potato. View Full-Text
Keywords: sweet potato; bacterial communities; nitrogen fixation; phosphate mineralization; plant genotype sweet potato; bacterial communities; nitrogen fixation; phosphate mineralization; plant genotype
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MDPI and ACS Style

Marques, J.M.; Mateus, J.R.; da Silva, T.F.; Couto, C.R.A.; Blank, A.F.; Seldin, L. Nitrogen Fixing and Phosphate Mineralizing Bacterial Communities in Sweet Potato Rhizosphere Show a Genotype-Dependent Distribution. Diversity 2019, 11, 231.

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