Impact of Land Use on Bacterial Diversity and Community Structure in Temperate Pine and Indigenous Forest Soils
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description and Soil Sampling
2.2. Analyses of Soil Properties
2.3. Determination of Relative Bacterial Diversity and Taxonomic Richness
2.4. Statistical Analyses
3. Results
3.1. Soil Properties (Physical and Chemical) of the Forest Soil Samples
3.2. Rarefaction Analysis
3.3. Assessment of Diversity Indices
3.4. Principal Coordinates Analysis
3.5. Phylum and Genus Level Distributions of the Bacterial Communities
3.6. Influence of Environmental Factors on Bacterial Communities
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | TC | TI | WC | WI |
---|---|---|---|---|
Organic C (%) | 3.21 ± 0.06 | 4.08 ± 0.07 | 2.97 ± 0.05 | 2.67 ± 0.07 |
NO3− (mg/kg) | 63.38 ± 0.16 | 65.13 ± 0.01 | 22.01 ± 0.19 | 5.91 ± 0.03 |
Total C (%) | 3.97 ± 0.05 | 4.58 ± 0.02 | 3.04 ± 0.00 | 2.91 ± 0.03 |
Total N (%) | 0.01 ± 0.00 | 0.02 ± 0.01 | 0.01 ± 0.00 | 0.001 ± 0.00 |
pH | 4.28 ± 0.01 | 4.78 ± 0.11 | 5.31 ± 0.03 | 5.10 ± 0.01 |
P (mg/kg) | 3.16 ± 0.00 | 2.33 ± 0.02 | 3.21 ± 0.08 | 5.03 ± 0.01 |
Ca (mg/kg) | 15.85 ± 0.05 | 234.50 ± 1.50 | 320.50 ± 1.50 | 164.50 ± 1.50 |
Mg (mg/kg) | 52.35 ± 0.15 | 95.60 ± 0.10 | 117.00 ± 1.00 | 97.65 ± 0.45 |
K (mg/kg) | 54.00 ± 0.10 | 70.30 ± 0.10 | 69.95 ± 0.35 | 92.20 ± 0.10 |
Na (mg/kg) | 11.60 ± 0.30 | 15.35 ± 0.15 | 16.60 ± 0.30 | 15.50 ± 0.20 |
Sand (%) | 23.50 ± 1.40 | 59.85 ± 3.25 | 55.15 ± 1.95 | 55.10 ± 0.60 |
Silt (%) | 50.80 ± 1.60 | 35.20 ± 2.10 | 17.45 ± 0.75 | 16.95 ± 0.45 |
Clay (%) | 19.00 ± 0.10 | 9.00 ± 0.60 | 23.45 ± 1.05 | 25.05 ± 0.75 |
Bioproject Number | SRR8135323 | SRR8134476 | SRR8136388 | SRR8136221 |
---|---|---|---|---|
Sampling site | TC | TI | WC | WI |
Uploading Information | ||||
bp count | 41,707,827 | 46,709,377 | 43,701,871 | 66,310,459 |
Sequences count | 91,160 | 101,138 | 95,076 | 144,319 |
Mean sequence length (bp) | 458 ± 14 | 458 ± 14 | 460 ± 15 | 459 ± 14 |
Mean GC content (%) | 57 ± 2 | 57 ± 3 | 56 ± 3 | 56 ± 2 |
Post QC Information | ||||
bp count | 5,310,782 | 6,752,690 | 6,060,310 | 7,440,367 |
Sequences count | 11,570 | 14,666 | 13,134 | 16,140 |
Mean sequence length (bp) | 459 ± 12 | 460 ± 12 | 461 ± 12 | 461 ± 11 |
Mean GC content (%) | 57 ± 3 | 57 ± 3 | 56 ± 3 | 56 ± 3 |
Processed Sequences | ||||
Predicted protein features | 93 | 85 | 65 | 79 |
Predicted rRNA features | 14,918 | 22,226 | 16,029 | 26,074 |
Aligned Sequences | ||||
Identified protein features | 28 | 27 | 19 | 19 |
Identified rRNA features | 13,720 | 21,040 | 15,252 | 24,702 |
Shannon_H | 1.95 | 1.98 | 1.88 | 1.95 |
Evenness_e^H/S | 1.31 | 1.31 | 1.36 | 1.62 |
Environmental variable | Explains % | Contribution % | Pseudo-F | P |
---|---|---|---|---|
Total C | 52.30 | 52.30 | 2.20 | 0.18 |
P | 36.80 | 36.80 | 3.40 | 0.22 |
pH | 11.00 | 11.00 | <0.1 | 1.00 |
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Amoo, A.E.; Babalola, O.O. Impact of Land Use on Bacterial Diversity and Community Structure in Temperate Pine and Indigenous Forest Soils. Diversity 2019, 11, 217. https://doi.org/10.3390/d11110217
Amoo AE, Babalola OO. Impact of Land Use on Bacterial Diversity and Community Structure in Temperate Pine and Indigenous Forest Soils. Diversity. 2019; 11(11):217. https://doi.org/10.3390/d11110217
Chicago/Turabian StyleAmoo, Adenike Eunice, and Olubukola Oluranti Babalola. 2019. "Impact of Land Use on Bacterial Diversity and Community Structure in Temperate Pine and Indigenous Forest Soils" Diversity 11, no. 11: 217. https://doi.org/10.3390/d11110217