Next Article in Journal
Beyond Capricornia: Tropical Sea Slugs (Gastropoda, Heterobranchia) Extend Their Distributions into the Tasman Sea
Next Article in Special Issue
DNA Barcoding and Taxonomic Challenges in Describing New Putative Species: Examples from Sootywing and Cloudywing Butterflies (Lepidoptera: Hesperiidae)
Previous Article in Journal
Changes in Species Composition of Birds and Declining Number of Breeding Territories over 40 Years in a Nature Conservation Area in Southwest Germany
Previous Article in Special Issue
DNA Barcoding Highlights Cryptic Diversity in the New Zealand Psylloidea (Hemiptera: Sternorrhyncha)
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessArticle
Diversity 2018, 10(3), 98; https://doi.org/10.3390/d10030098

cpDNA Barcoding by Combined End-Point and Real-Time PCR Analyses to Identify and Quantify the Main Contaminants of Oregano (Origanum vulgare L.) in Commercial Batches

Laboratory of Genomics for Breeding, Department of Agronomy, Food, Natural Resources, Animals and Environment, DAFNAE, University of Padova, Agripolis—Legnaro, 35020 Padova, Italy
*
Author to whom correspondence should be addressed.
Received: 22 July 2018 / Revised: 20 August 2018 / Accepted: 28 August 2018 / Published: 4 September 2018
(This article belongs to the Special Issue DNA Barcoding for Biodiversity)
Full-Text   |   PDF [1855 KB, uploaded 4 September 2018]   |  

Abstract

Oregano (Origanum vulgare L.) is a flowering plant that belongs to the mint family (Lamiaceae). It is used as a culinary herb and is often commercialized as a fine powder or a mixture of small fragments of dried leaves, which makes morphological recognition difficult. Like other commercial preparations of drugs and spices, the contamination of oregano mixtures with vegetable matter of lower quality, or the use of generic misleading names, are frequent and stress the need to develop a molecular traceability system to easily, quickly, and cheaply unveil these scams. The DNA-based analytical approach known as cpDNA barcoding is particularly suited for fraud identification in crop plant species (fresh products and food derivatives), and it represents a promising traceability tool as an alternative or complement to traditional detection methods. In the present study, we used a combined approach based on both qualitative and quantitative cpDNA barcoding with end-point and real-time polymerase chain reaction (PCR) analyses to assess the type and degree of contamination in commercial batches of common oregano. In a preliminary qualitative screening, we amplified, cloned, and sequenced a number of universal trnH-psbA- and trnL-barcoded regions, to identify the main contaminants in the samples under investigation. On the basis of these findings, we then developed and validated a species-specific and sequence-targeted method of testing for the quantitative assessment of contaminants, using trnL gene intron assays. Surprisingly, the results obtained in our case study indicated an almost total absence of O. vulgare in the commercial batches analyzed, but a high presence of group I contaminants (Satureja pilosa Velen.), and a moderate presence of group II contaminants (Cistus lanidifer L./Cistus albidus). View Full-Text
Keywords: spices; oregano; species authentication; food traceability; DNA barcodes spices; oregano; species authentication; food traceability; DNA barcodes
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Vannozzi, A.; Lucchin, M.; Barcaccia, G. cpDNA Barcoding by Combined End-Point and Real-Time PCR Analyses to Identify and Quantify the Main Contaminants of Oregano (Origanum vulgare L.) in Commercial Batches. Diversity 2018, 10, 98.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Diversity EISSN 1424-2818 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top