Establishment of Autoreactive CD4+CD8+ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice

Mashimo, Shuhei; Arita, Michitsune; Kuwabara, Taku; Naito, Taku; Takizawa, Sakurako; Inoue, Akiko; Ishiko, Akira; Kondo, Motonari; Tanaka, Yuriko

doi:10.3390/ijms27010414

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice

by

Shuhei Mashimo

^1,2,3,

Michitsune Arita

¹,

Taku Kuwabara

¹

,

Taku Naito

¹,

Sakurako Takizawa

¹,

Akiko Inoue

⁴

,

Akira Ishiko

²

,

Motonari Kondo

^1,*

and

Yuriko Tanaka

¹

Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan

²

Department of Dermatology, Toho University Omori Medical Center, Tokyo 143-8541, Japan

³

Graduate School of Medicine, Toho University, Tokyo 143-8540, Japan

⁴

Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo 143-8541, Japan

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2026, 27(1), 414; https://doi.org/10.3390/ijms27010414 (registering DOI)

Submission received: 20 November 2025 / Revised: 26 December 2025 / Accepted: 26 December 2025 / Published: 30 December 2025

(This article belongs to the Special Issue Autoimmune Diseases: A Swing Dance of Immune Cells, 3rd Edition)

Download Review Reports Versions Notes

Abstract

Sjögren’s disease (SjD), which is also known as Sjögren’s syndrome (SS), is a chronic autoimmune disease characterized by dysfunction of exocrine glands, such as the salivary and lacrimal glands, resulting in xerostomia (dry mouth) and keratoconjunctivitis sicca (dry eyes). Mice in which the SATB1 gene is conditionally deleted in hematopoietic cells (SATB1cKO mice) develop SS as early as 4 weeks of age; however, the etiology of the disease remains to be elucidated. Here, we found that the frequency of abnormally appearing CD4⁺CD8⁺ double positive (DP) T cells in the periphery of SATB1cKO mice was higher in the salivary glands than that in the spleen, suggesting a possible involvement of DP T cells in the pathogenesis of SS in SATB1cKO mice. To investigate the nature of DP T cells, we established DP T cell hybridomas by fusing T cells from the cervical lymph nodes of SATB1cKO mice with the BW5147 thymoma cell line. Among six DP hybridoma clones, the TCRβ gene from five clones exhibited a fetal or immature phenotype. In addition, four out of five clones exhibited upregulated transcription of IL-2 in the salivary glands of T/B cell-deficient RAG2 ^−/− mice, suggesting that autoreactive T cells were enriched in the DP T cell population of SATB1cKO mice. These results suggest that unusual DP T cells in SATB1cKO mice may be involved in autoimmune pathogenesis in SATB1cKO mice.

Keywords: autoimmunity; Sjögren’s disease; T cell hybridoma; T cell receptor; complementarity determining region 3

Share and Cite

MDPI and ACS Style

Mashimo, S.; Arita, M.; Kuwabara, T.; Naito, T.; Takizawa, S.; Inoue, A.; Ishiko, A.; Kondo, M.; Tanaka, Y. Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice. Int. J. Mol. Sci. 2026, 27, 414. https://doi.org/10.3390/ijms27010414

AMA Style

Mashimo S, Arita M, Kuwabara T, Naito T, Takizawa S, Inoue A, Ishiko A, Kondo M, Tanaka Y. Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice. International Journal of Molecular Sciences. 2026; 27(1):414. https://doi.org/10.3390/ijms27010414

Chicago/Turabian Style

Mashimo, Shuhei, Michitsune Arita, Taku Kuwabara, Taku Naito, Sakurako Takizawa, Akiko Inoue, Akira Ishiko, Motonari Kondo, and Yuriko Tanaka. 2026. "Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice" International Journal of Molecular Sciences 27, no. 1: 414. https://doi.org/10.3390/ijms27010414

APA Style

Mashimo, S., Arita, M., Kuwabara, T., Naito, T., Takizawa, S., Inoue, A., Ishiko, A., Kondo, M., & Tanaka, Y. (2026). Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice. International Journal of Molecular Sciences, 27(1), 414. https://doi.org/10.3390/ijms27010414

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.

Article Menu

Establishment of Autoreactive CD4⁺CD8⁺ T Cell Hybridomas from Sjögren’s Disease Model, SATB1 Conditional Knockout Mice

Abstract

Share and Cite

Article Metrics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI