In the original publication [1], there were errors in the +miR-29b mimic (α-SMA) in Figure 2f and +miR-29b (α-SMA) Control in Figure 3f; they were repeated. It appears that the α-SMA and E-cad data in Figure 2 were mistakenly left as temporary data and should have been updated. However, only the HSP data was corrected, while the α-SMA data was inadvertently not updated. And the labels in Figures 2c and 3c are incorrect. Figure 2c should be labeled as miR-29b mimic. Figure 3c should be labeled as miR-29b inhibitor. The corrected Figure 2c,f and Figure 3c appear below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Figure 2.
Overexpression of miR-29b inhibited mRNA and protein expression levels of TGF-β1-induced EMT markers in A549 cells. A549 cells were stimulated with TGF-β1 (5 ng/mL) with miR control or miR-29b mimic. (a,b) mRNA expression levels of miR-29b and HSP47 were determined using qPCR. (c) HSP47 luciferase activity was measured by luciferase assay. (d) E-cadherin, α-SMA, vimentin, and fibronectin mRNA levels were analyzed through qPCR. (e) Protein expression levels of HSP47, E-cadherin, α-SMA, vimentin, and fibronectin were determined using Western blotting. (f) The cells were treated with TGF-β1 for 72 h after transfection of miR-29b mimic and then assessed for HSP47 (1st line, green), vimentin (1st line, red), α-SMA (2nd, green), and E-cadherin (2nd, red) expression/localization using immunofluorescence. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. Values are expressed as mean ± SEM of three independent samples. * p < 0.05, vs. control + miR Control; † p < 0.05, vs. TGF-β1 + miR Control.
Figure 3.
Inhibition of miR-29b expression induced mRNA and protein expression levels of TGF-β1-induced EMT markers in A549 cells. A549 cells were stimulated with TGF-β1 (5 ng/mL) with miR control or a miR-29b inhibitor. (a,b) The mRNA expression levels of miR-29b and HSP47 were analyzed using qPCR. (c) HSP47 luciferase activity was measured by luciferase assay. (d) The mRNA levels of EMT-related markers were measured using qPCR. (e) Protein expression levels of HSP47, E-cadherin, α-SMA, vimentin and fibronectin were determined using Western blotting. (f) The cells were treated with TGF-β1 for 72 h after transfection with miR-29b inhibitor, and then assessed for HSP47 (1st line, green), vimentin (1st line, red), α-SMA (2nd, green), and E-cadherin (2nd, red) expression/localization using immunofluorescence. Nuclei were stained with DAPI (blue). Scale bar = 20 μm. Values are expressed as mean ± SEM of three independent samples. * p < 0.05 vs. control + miR Control; † p < 0.05, vs. TGF-β1 + miR Control.
Reference
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