Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling

Le, Ducdat; Dang, Thinhulinh; Truong, Vinhquang; Dinh, Thientam; Yu, Soojung; Lee, Seok-Geun; Lee, Mina

doi:10.3390/ijms26178421

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Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling

by

Ducdat Le

^1,2,†

,

Thinhulinh Dang

^1,†,

Vinhquang Truong

¹,

Thientam Dinh

¹,

Soojung Yu

²,

Seok-Geun Lee

^3,4

and

Mina Lee

^1,3,*

¹

College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Republic of Korea

²

Department of Natural Cosmetics Science and Smart Beautytech Research Institute, Sunchon National University, 255 Jungangno, Suncheon 57922, Republic of Korea

³

Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea

⁴

BioNanocomposite Research Center, Kyung Hee University, Seoul 02447, Republic of Korea

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2025, 26(17), 8421; https://doi.org/10.3390/ijms26178421

Submission received: 28 June 2025 / Revised: 21 August 2025 / Accepted: 26 August 2025 / Published: 29 August 2025

(This article belongs to the Special Issue Functions and Applications of Natural Products)

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Abstract

Osmanthus fragrans var. aurantiacus Makino is a traditional medicine for treating various diseases, including inflammation. In this study, we discovered the biological features of this plant by assessing antioxidative and anti-inflammatory activities. The GNPS-FBMN approach and in vitro assays guided the identification of active ingredients. As a result, one new compound and 17 other compounds were separated and identified. The structure of the new compound was established by CD spectrum and hydrolysis, followed by HPLC analysis. These compounds demonstrated antioxidative and anti-inflammatory activities. Western blotting clarified the active compound by inhibiting inflammation through COX-2 and iNOS enzymes and blocking the ERK 1/2 MAPK signaling. In silico approaches supported the binding affinity and dynamic features of the established complexes’ target inflammation. Our finding supports evidence from both experimental and in silico approaches that O. fragrans fractions and its constituents may be employed as potential therapeutic phytochemicals for treating inflammatory bowel diseases.

Keywords: Osmanthus fragrans; isolated compounds; inflammation; antioxidant; ERK 1/2 MAPK

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MDPI and ACS Style

Le, D.; Dang, T.; Truong, V.; Dinh, T.; Yu, S.; Lee, S.-G.; Lee, M. Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling. Int. J. Mol. Sci. 2025, 26, 8421. https://doi.org/10.3390/ijms26178421

AMA Style

Le D, Dang T, Truong V, Dinh T, Yu S, Lee S-G, Lee M. Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling. International Journal of Molecular Sciences. 2025; 26(17):8421. https://doi.org/10.3390/ijms26178421

Chicago/Turabian Style

Le, Ducdat, Thinhulinh Dang, Vinhquang Truong, Thientam Dinh, Soojung Yu, Seok-Geun Lee, and Mina Lee. 2025. "Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling" International Journal of Molecular Sciences 26, no. 17: 8421. https://doi.org/10.3390/ijms26178421

APA Style

Le, D., Dang, T., Truong, V., Dinh, T., Yu, S., Lee, S.-G., & Lee, M. (2025). Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling. International Journal of Molecular Sciences, 26(17), 8421. https://doi.org/10.3390/ijms26178421

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Chemical Constituents from Osmanthus fragrans var. aurantiacus Makino with Their In Vitro and In Silico Studies Target Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling

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