NMR Study of Water-Soluble Carotenoid Crocin: Formation of Mixed Micelles, Interaction with Lipid Membrane and Antioxidant Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. NMR Study of Crocin/GA Mixed Micelles in Water Solution
2.2. NMR and MD Study of Crocin Interaction with LA Micelles and DMPC/DHPC Bicelles
2.3. Peroxidation of Linoleic Acid Micelles in the Presence of Crocin
LOO + LOO → LOOL + O2
3. Materials and Methods
3.1. Materials
3.2. NMR and Selective NOESY Measurements
3.3. Sample Preparation for Lipid Peroxidation Studies
3.4. Sample Preparation for NMR Study of Crocin Interaction with Bicells
3.5. Molecular Dynamics Simulations
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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System | Rate Constant, in 10−5 s−1 |
---|---|
LA | 17 ± 1 |
LA + 1 mM crocin | 2.9 ± 0.2 |
LA + 1 mM Na2GA | 1.8 ± 0.2 |
LA + 0.1 mM crocin + 1 mM Na2GA | 1.8 ± 0.2 |
LA + 2.5 mM ascorbate | 128 ± 4 |
LA + 1 mM crocin + ascorbate | 10.7 ± 0.3 |
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Su, W.; Mastova, A.V.; Ul’yanova, M.A.; Kononova, P.A.; Selyutina, O.Y.; Evseenko, V.I.; Meteleva, E.S.; Dushkin, A.V.; Su, W.; Polyakov, N.E. NMR Study of Water-Soluble Carotenoid Crocin: Formation of Mixed Micelles, Interaction with Lipid Membrane and Antioxidant Activity. Int. J. Mol. Sci. 2024, 25, 3194. https://doi.org/10.3390/ijms25063194
Su W, Mastova AV, Ul’yanova MA, Kononova PA, Selyutina OY, Evseenko VI, Meteleva ES, Dushkin AV, Su W, Polyakov NE. NMR Study of Water-Soluble Carotenoid Crocin: Formation of Mixed Micelles, Interaction with Lipid Membrane and Antioxidant Activity. International Journal of Molecular Sciences. 2024; 25(6):3194. https://doi.org/10.3390/ijms25063194
Chicago/Turabian StyleSu, Wenjing, Anna V. Mastova, Maya A. Ul’yanova, Polina A. Kononova, Olga Yu. Selyutina, Veronika I. Evseenko, Elizaveta S. Meteleva, Alexander V. Dushkin, Weike Su, and Nikolay E. Polyakov. 2024. "NMR Study of Water-Soluble Carotenoid Crocin: Formation of Mixed Micelles, Interaction with Lipid Membrane and Antioxidant Activity" International Journal of Molecular Sciences 25, no. 6: 3194. https://doi.org/10.3390/ijms25063194