Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis
Abstract
:1. Introduction
2. Result
2.1. Quercetin Could Upregulate Wnt/β-Catenin and Downregulate NF-κB Signaling
2.2. Quercetin Rescued TNFα-Impaired BMSCs Osteogenesis via Increasing Wnt/β-Catenin and Decreasing NF-κB Signaling
2.3. Quercetin Could Reverse the Expression of lncRNA Malat1 Downregulated by TNFα Treatment
2.4. Knockdown of Malat1 Ameliorated the Rescuing Effect of Quercetin on TNFα-Impaired BMSCs Osteogenesis
2.5. Quercetin Attenuates Bone Loss in the OVX Mouse Osteoporosis Model via Upregulating Malat1 Expression
3. Discussion
4. Material and Methods
4.1. Herb-Derived Small Molecule Library
4.2. Transient Cell Transfection and Dual-Luciferase Reporter Assay
4.3. Cell Viability Assay
4.4. Osteogenesis Induction
4.5. RNA Isolation and Real-Time PCR
4.6. Western Blot
4.7. Ovariectomized Osteoporotic Model Mice Experiment
4.8. Micro-Computed Tomography Analysis
4.9. Immunohistochemistry and Histology
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Feng, L.; Yang, Z.; Hou, N.; Wang, M.; Lu, X.; Li, Y.; Wang, H.; Wang, Y.; Bai, S.; Zhang, X.; et al. Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis. Int. J. Mol. Sci. 2023, 24, 5965. https://doi.org/10.3390/ijms24065965
Feng L, Yang Z, Hou N, Wang M, Lu X, Li Y, Wang H, Wang Y, Bai S, Zhang X, et al. Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis. International Journal of Molecular Sciences. 2023; 24(6):5965. https://doi.org/10.3390/ijms24065965
Chicago/Turabian StyleFeng, Lu, Zhengmeng Yang, Nan Hou, Ming Wang, Xuan Lu, Yucong Li, Haixing Wang, Yaofeng Wang, Shanshan Bai, Xiaoting Zhang, and et al. 2023. "Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis" International Journal of Molecular Sciences 24, no. 6: 5965. https://doi.org/10.3390/ijms24065965