Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis
Abstract
:1. Introduction
2. Result
2.1. Quercetin Could Upregulate Wnt/β-Catenin and Downregulate NF-κB Signaling
2.2. Quercetin Rescued TNFα-Impaired BMSCs Osteogenesis via Increasing Wnt/β-Catenin and Decreasing NF-κB Signaling
2.3. Quercetin Could Reverse the Expression of lncRNA Malat1 Downregulated by TNFα Treatment
2.4. Knockdown of Malat1 Ameliorated the Rescuing Effect of Quercetin on TNFα-Impaired BMSCs Osteogenesis
2.5. Quercetin Attenuates Bone Loss in the OVX Mouse Osteoporosis Model via Upregulating Malat1 Expression
3. Discussion
4. Material and Methods
4.1. Herb-Derived Small Molecule Library
4.2. Transient Cell Transfection and Dual-Luciferase Reporter Assay
4.3. Cell Viability Assay
4.4. Osteogenesis Induction
4.5. RNA Isolation and Real-Time PCR
4.6. Western Blot
4.7. Ovariectomized Osteoporotic Model Mice Experiment
4.8. Micro-Computed Tomography Analysis
4.9. Immunohistochemistry and Histology
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Feng, L.; Yang, Z.; Hou, N.; Wang, M.; Lu, X.; Li, Y.; Wang, H.; Wang, Y.; Bai, S.; Zhang, X.; Lin, Y.; Yan, X.; Lin, S.; Tortorella, M.D.; Li, G. Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis. Int. J. Mol. Sci. 2023, 24, 5965. https://doi.org/10.3390/ijms24065965
Feng L, Yang Z, Hou N, Wang M, Lu X, Li Y, Wang H, Wang Y, Bai S, Zhang X, Lin Y, Yan X, Lin S, Tortorella MD, Li G. Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis. International Journal of Molecular Sciences. 2023; 24(6):5965. https://doi.org/10.3390/ijms24065965
Chicago/Turabian StyleFeng, Lu, Zhengmeng Yang, Nan Hou, Ming Wang, Xuan Lu, Yucong Li, Haixing Wang, Yaofeng Wang, Shanshan Bai, Xiaoting Zhang, Yuejun Lin, Xu Yan, Sien Lin, Micky D. Tortorella, and Gang Li. 2023. "Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis" International Journal of Molecular Sciences 24, no. 6: 5965. https://doi.org/10.3390/ijms24065965