Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity of DON, ZEA and Their Mixtures to HepG2 Cells
2.2. Genotoxic Activity of DON, ZEA and Their Mixtures in HepG2 Cells
2.3. Effect of DON, ZEA and Their Mixtures on Cell Cycle Progression of HepG2 Cells
2.4. Effect of DON, ZEA and Their Mixtures on the Proliferation of HepG2 Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals and Preparation of Mycotoxins’ Standard Solution
4.2. Cell Culture
4.3. Cytotoxicity—The MTS Assay
4.4. DNA Strand Breaks
4.5. Simultaneous Determination of the Gamma-H2AX Positive Cells, Cell Proliferation and Cell Cycle by Flow Cytometry
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Domijan, A.-M.; Hercog, K.; Štampar, M.; Gajski, G.; Gerić, M.; Sokolović, M.; Žegura, B. Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells. Int. J. Mol. Sci. 2023, 24, 4082. https://doi.org/10.3390/ijms24044082
Domijan A-M, Hercog K, Štampar M, Gajski G, Gerić M, Sokolović M, Žegura B. Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells. International Journal of Molecular Sciences. 2023; 24(4):4082. https://doi.org/10.3390/ijms24044082
Chicago/Turabian StyleDomijan, Ana-Marija, Klara Hercog, Martina Štampar, Goran Gajski, Marko Gerić, Marijana Sokolović, and Bojana Žegura. 2023. "Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells" International Journal of Molecular Sciences 24, no. 4: 4082. https://doi.org/10.3390/ijms24044082