The Effects of Sirolimus and Magnesium on Primary Human Coronary Endothelial Cells: An In Vitro Study
Abstract
:1. Introduction
2. Results
2.1. The Effects of Mg and SIR on hCAEC Viability
2.2. The Effects of Mg and SIR on hCAEC Cytoskeletal Organization and Mg2+ Intracellular Content
2.3. The Effects of Mg and SIR on hCAEC Migration
2.4. The Effects of Mg and SIR on the Interaction between hCAEC and Monocyte-Like Cells
2.5. The Effects of Mg and SIR on Endothelial Permeability
2.6. The Effects of Mg and SIR on the Localization of ZO-1 and VE-CAD
2.7. The Effects of Mg and SIR on Nitric Oxide Production
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Viability Assay
4.3. Intracellular Mg2+ Quantification
4.4. In Vitro Wound Assay
4.5. Adhesion Assay
4.6. Confocal Imaging
4.7. Enzyme-Linked Immunosorbent Assay–ELISA
4.8. Western Blot
4.9. X-PerT Assay
4.10. Measurement of Released NO
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fedele, G.; Castiglioni, S.; Maier, J.A.M.; Locatelli, L. The Effects of Sirolimus and Magnesium on Primary Human Coronary Endothelial Cells: An In Vitro Study. Int. J. Mol. Sci. 2023, 24, 2930. https://doi.org/10.3390/ijms24032930
Fedele G, Castiglioni S, Maier JAM, Locatelli L. The Effects of Sirolimus and Magnesium on Primary Human Coronary Endothelial Cells: An In Vitro Study. International Journal of Molecular Sciences. 2023; 24(3):2930. https://doi.org/10.3390/ijms24032930
Chicago/Turabian StyleFedele, Giorgia, Sara Castiglioni, Jeanette A. M. Maier, and Laura Locatelli. 2023. "The Effects of Sirolimus and Magnesium on Primary Human Coronary Endothelial Cells: An In Vitro Study" International Journal of Molecular Sciences 24, no. 3: 2930. https://doi.org/10.3390/ijms24032930