Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage
Abstract
:1. Introduction
2. Results
2.1. Melatonin Delaying Leaf Senescence was Associated with Chlorophyll Degradation, Ethylene Biosynthesis, and Flavonoid Accumulation
2.2. BrFLS1 and BrFLS3.2, Key Genes for Flavonoid Biosynthesis, Played a Positive Regulatory Role in Melatonin-Delayed Leaf Senescence
2.3. BrERF2 and BrERF109, the Transcriptional Suppressors, Were Involved in Leaf Senescence and Repressed by Melatonin
2.4. BrERF2 and BrERF109 Directly Bound to the Promoters of BrFLSs and Suppressed Their Expression
2.5. Silencing BrERF2 and BrERF109 Promoted Flavonoid Biosynthesis and Delayed Aging
2.6. BrERF2 Interacted with BrERF109, Which Formed a BrERF2-BrERF109 Complex
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Determination of Ethylene Content
4.3. Determination of Chlorophyll and Total Flavonoid Content
4.4. Total RNA Extraction, Quantitative Real-Time PCR (qRT-PCR) and Sequence Analysis
4.5. Virus-Induced Gene Silencing (VIGS)
4.6. Subcellular Localization Analysis
4.7. Transcriptional Assay
4.8. Yeast One-Hybrid (Y1H) Assay
4.9. β-Glucuronidase (GUS) Analysis
4.10. Dual Luciferase (LUC) Assay
4.11. Y2H Screening
4.12. Bimolecular Fluorescence Complementation (BiFC) Assay
4.13. Statistical Analysis
4.14. Primers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yue, L.; Kang, Y.; Zhong, M.; Kang, D.; Zhao, P.; Chai, X.; Yang, X. Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage. Int. J. Mol. Sci. 2023, 24, 2933. https://doi.org/10.3390/ijms24032933
Yue L, Kang Y, Zhong M, Kang D, Zhao P, Chai X, Yang X. Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage. International Journal of Molecular Sciences. 2023; 24(3):2933. https://doi.org/10.3390/ijms24032933
Chicago/Turabian StyleYue, Lingqi, Yunyan Kang, Min Zhong, Dengjin Kang, Puyan Zhao, Xirong Chai, and Xian Yang. 2023. "Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage" International Journal of Molecular Sciences 24, no. 3: 2933. https://doi.org/10.3390/ijms24032933