Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing

Wang, Weiling; Zhang, Yuting; Liu, Chang; Dong, Yongwen; Jiang, Xue; Zhao, Can; Li, Guohui; Xu, Ke; Huo, Zhongyang

doi:10.3390/ijms242115892

Open AccessArticle

Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing

by

Weiling Wang

,

Yuting Zhang

,

Chang Liu

,

Yongwen Dong

,

Xue Jiang

,

Can Zhao

,

Guohui Li

,

Ke Xu

and

Zhongyang Huo

^*

Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, China

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2023, 24(21), 15892; https://doi.org/10.3390/ijms242115892

Submission received: 5 September 2023 / Revised: 20 October 2023 / Accepted: 23 October 2023 / Published: 2 November 2023

(This article belongs to the Special Issue Plant Stress-Induced Responses and Tolerance Mechanisms at Biochemical, Cellular, Physiological and Molecular Levels)

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Abstract

Late spring frost is an important meteorological factor threatening the safe production of winter wheat in China. The young ear is the most vulnerable organ of the wheat plant to spring frost. To gain an insight into the mechanisms underpinning young wheat ears’ tolerance to freezing, we performed a comparative proteome analysis of wheat varieties Xumai33 (XM33, freezing-sensitive) and Jimai22 (JM22, freezing-tolerant) under normal and freezing conditions using label-free quantitative proteomic techniques during the anther connective tissue formation phase (ACFP). Under freezing stress, 392 and 103 differently expressed proteins (DEPs) were identified in the young ears of XM33 and JM22, respectively, and among these, 30 proteins were common in both varieties. A functional characterization analysis revealed that these DEPs were associated with antioxidant capacity, cell wall modification, protein folding, dehydration response, and plant–pathogen interactions. The young ears of JM22 showed significantly higher expression levels of antioxidant enzymes, heat shock proteins, and dehydrin under normal conditions compared to those of XM33, which might help to prepare the young ears of JM22 for freezing stress. Our results lead to new insights into understanding the mechanisms in young wheat ears’ response to freezing stress and provide pivotal potential candidate proteins required for improving young wheat ears’ tolerance to spring frost.

Keywords: antioxidant capacity; cell wall modification; heat shock protein; dehydrin; defensin

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MDPI and ACS Style

Wang, W.; Zhang, Y.; Liu, C.; Dong, Y.; Jiang, X.; Zhao, C.; Li, G.; Xu, K.; Huo, Z. Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing. Int. J. Mol. Sci. 2023, 24, 15892. https://doi.org/10.3390/ijms242115892

AMA Style

Wang W, Zhang Y, Liu C, Dong Y, Jiang X, Zhao C, Li G, Xu K, Huo Z. Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing. International Journal of Molecular Sciences. 2023; 24(21):15892. https://doi.org/10.3390/ijms242115892

Chicago/Turabian Style

Wang, Weiling, Yuting Zhang, Chang Liu, Yongwen Dong, Xue Jiang, Can Zhao, Guohui Li, Ke Xu, and Zhongyang Huo. 2023. "Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing" International Journal of Molecular Sciences 24, no. 21: 15892. https://doi.org/10.3390/ijms242115892

APA Style

Wang, W., Zhang, Y., Liu, C., Dong, Y., Jiang, X., Zhao, C., Li, G., Xu, K., & Huo, Z. (2023). Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing. International Journal of Molecular Sciences, 24(21), 15892. https://doi.org/10.3390/ijms242115892

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Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing

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