Bacillus amyloliquefaciens AK-12 Helps Rapeseed Establish a Protection against Brevicoryne brassicae
Abstract
:1. Introduction
2. Results
2.1. Comparison of Strain Stability and Growth Curves
2.2. Colonization Dynamics of AK-12
2.3. Strain Colonization Ability to Establish Green Barrier and Control Cabbage Aphids
2.3.1. Effect of Strains on Cabbage Aphid Transmission
2.3.2. Effect of AK-12 on Cabbage Aphid Feeding
2.4. Growth Promoting Effect of AK-12 on Rapeseed Plants
2.5. Induced Resistance Studies of Strain
3. Discussion
4. Materials and Methods
4.1. Materials, Bacterial Strains, and Reagents
4.2. Natural Transformation of GFP-Harboring Vactor in the Bacterial Strains
4.3. Comparison of Strain Stability and Growth Curves
4.4. External and Internal Colonization of AK-12 in Rapeseed Leaves
4.5. AK-12 Colonization Ability to Establish Green Barrier against Aphids
4.5.1. Effect of AK-12 on Aphid Transmission
4.5.2. Effect of Strains on Aphid Feeding
4.6. Growth Promotion in Rapeseed Treated with AK-12
4.7. Study of Induced Resistance in Rapeseed Due to AK-12
4.8. Data Statistics and Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Stem Length (cm) | Stem Thick (mm) | Fresh Weight (g) | Dry Weight (g) | |
---|---|---|---|---|
Foliar spray | 27.92 ± 1.98 ab | 3.62 ± 0.29 ab | 4.06 ± 0.51 ab | 0.46 ± 0.12 ab |
Irrigation | 30.7 ± 2.68 a | 4.06 ± 0.31 a | 5.17 ± 0.63 a | 0.59 ± 0.06 a |
Foliar spraying and irrigation | 29.82 ± 1.81 a | 4.08 ± 0.32 a | 5.5 ± 1.49 a | 0.65 ± 0.17 a |
CK | 24.6 ± 0.76 b | 3.08 ± 0.28 b | 2.69 ± 0.20 b | 0.28 ± 0.05 b |
Primers | Primer-F Sequence (5′-3′) | Primers-R Sequence (5′-3′) |
---|---|---|
Tubulin | GAGCGACCCACATACACCAATC | AACCTCAACGAAGCAGTCAACG |
NPR1 | ACGCTTCTTTCCACGATGTTCAG | GCTTCTTCAGTTGACGCTCTTCC |
PDF1.2 | GGGACCATGCTCAAGAGACAG | AACAACGGCGGCGGAATC |
chit | TCGGCAGTATCATCTCAAGTTCC | TTTACGGGCAGTGGTATCGC |
POD | ACACACATTTGGAAGAGCAAGATG | CGTCTACGGTTGGATCAGGATTAC |
PPO | TGGGTTTAGGAGGGCTGTATGG | TGAGATCAGGAGGTGGTATAGGAG |
PAL | CGGTTTGCCCTCTAATCTCACTG | GACATCTTGGTTGTGTTGTTCAGC |
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Qian, S.; Ahmed, A.; He, P.; He, P.; Munir, S.; Xia, M.; Tang, C.; Tang, P.; Wang, Z.; Khan, R.; et al. Bacillus amyloliquefaciens AK-12 Helps Rapeseed Establish a Protection against Brevicoryne brassicae. Int. J. Mol. Sci. 2023, 24, 15893. https://doi.org/10.3390/ijms242115893
Qian S, Ahmed A, He P, He P, Munir S, Xia M, Tang C, Tang P, Wang Z, Khan R, et al. Bacillus amyloliquefaciens AK-12 Helps Rapeseed Establish a Protection against Brevicoryne brassicae. International Journal of Molecular Sciences. 2023; 24(21):15893. https://doi.org/10.3390/ijms242115893
Chicago/Turabian StyleQian, Shixiong, Ayesha Ahmed, Pengbo He, Pengfei He, Shahzad Munir, Mengyuan Xia, Chaoyun Tang, Ping Tang, Zaiqiang Wang, Rizwan Khan, and et al. 2023. "Bacillus amyloliquefaciens AK-12 Helps Rapeseed Establish a Protection against Brevicoryne brassicae" International Journal of Molecular Sciences 24, no. 21: 15893. https://doi.org/10.3390/ijms242115893